DOI QR코드

DOI QR Code

MHC Class II+ (HLA-DP-like) Cells in the Cow Reproductive Tract: I. Immunolocalization and Distribution of MHC Class II+ Cells in Uterus at Different Phases of the Estrous Cycle

  • Eren, U. ;
  • Sandikci, M. ;
  • Kum, S. ;
  • Eren, V.
  • Received : 2007.04.22
  • Accepted : 2007.08.14
  • Published : 2008.01.01

Abstract

This study was undertaken to investigate the distribution of major histocompatibility complex class II positive (MHC II+) (HLA-DP-like) cells in the cow uterus (cervix, corpus and cornu uteri) and to compare these cells between the estrus and diestrus phases of the estrous cycle. Twenty-nine multiparous cows were used. Tissue samples from the middle of the cervix, the corpus and the right cornu were taken immediately after slaughter at the estrus or diestrus phase. Streptavidin-biotin peroxidase complex staining was used to detect MHC II+ cells. The number of MHC II+ cells per unit area of tissue was counted using image analysis software under a light microscope. Numerous MHC II+ cells were found in the endometrium (cervix, corpus and cornu uteri) in both estrus and diestrus. MHC II+ cells were found in the surface epithelium of the cervix uteri in diestrus, but in the corpus uteri in both estrus and diestrus and in the cornu uteri in estrus. MHC II+ cells were also found freely in the lumen of the glands and between the gland epithelia of the corpus and cornu uteri in both estrus and diestrus. There were also MHC II+ cells in the connective tissue of the myometrium and perimetrium (outside the endometrium) and around the blood vessels. Endothelial cells were frequently positive for MHC II staining. More MHC II+ cells were found in the endometrium than outside the endometrium in both estrus and diestrus (p<0.001). However, there was no difference in the numbers of positive cells between estrus and diestrus either in the endometrium or outside it. These results are the first evidence for HLA-DP-like MHC II+ cells in the bovine uterus. They indicate that antigen presentation by HLA-DP-like MHC II+ cells of the uterus is not influenced by hormonal status.

Keywords

MHC Class II;Cow;Genital Tract;Estrous Cycle

References

  1. Arthur, G. H., E. D. Nonkes and H. Pearson. 1996. Veterinary reproduction and obstetrics. WB Sounders Company Ltd, pp. 17-27.
  2. Ababou, A., F. Femenia, F. Crespeau, J. J. Fontaine, W. C. Davis and D. Levy. 1995. A novel BoLA class II molecule with a tissue distribution different from BoLA-DR or BoLA-DQ molecules. Vet. Res. 26:319-327.
  3. Ababou, A., J. Goyeneche, W. C. Davis and D. Levy. 1994. Evidence for the expression of three different BoLA-class II non-DR non-DQ gene product. J. Leukoc. Biol. 56:182-186. https://doi.org/10.1002/jlb.56.2.182
  4. German, A. J., E. J. Hall and M. J. Day. 1999. Analysis of leucocyte subsets in the canine intestine. J. Comp. Pathol. 120:129-145. https://doi.org/10.1053/jcpa.1998.0262
  5. Head, J. R. and S. D. Gaede. 1986. Ia antigen expression in the rat uterus. J. Reprod. Immunol. 9:137-153. https://doi.org/10.1016/0165-0378(86)90007-0
  6. Henz, B. M., M. Maurer, U. Lippert, M. Worm and M. Babina. 2001. Mast cells as initiators of immunity and host defense. Exp. Dermatol. 10:1-10. https://doi.org/10.1034/j.1600-0625.2001.100101.x
  7. Hsu, S. M., L. Raine and H. Fanger. 1981. Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparision between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-580. https://doi.org/10.1177/29.4.6166661
  8. http://www.vmrd.com/docs/ab/ihc.htm, VMRD ABC technique. 01 Oct. 2001.
  9. Johansson, E. L., A. Rudin, L. Wassen and J. Holmren. 1999. Distribution of lymphocytes and adhesion molecules in human cervix and vagina. Immunol. 96:272-277. https://doi.org/10.1046/j.1365-2567.1999.00675.x
  10. Canning, M. B. and W. D. Billington. 1983. Hormonal regulation of immunoglobulins and plasma cells in the mouse uterus. J. Endocrinol. 97:419-424. https://doi.org/10.1677/joe.0.0970419
  11. Cobb, S. P. and E. D. Watson. 1995. Immunohistochemical study of immune cells in the bovine endometrium at different stages of the oestrous cycle. Res. Vet. Sci. 59:238-241. https://doi.org/10.1016/0034-5288(95)90010-1
  12. De, M. and. G. W. Wood. 1990. Influence of oestrogen and progesterone on macrophage distribution in the mouse uterus. J. Endocrinol. 126:417-424. https://doi.org/10.1677/joe.0.1260417
  13. Diker, S. 1998. lmmunoloji, Medisan Yayınevi, pp. 73-84.
  14. Donaldson, W. L., C. H. Zhang, J. G. Oriol and D. F. Antczak. 1990. Invasive equine trophoblast expresses conventional class I major histocompatibility complex antigens. Develop. 110:63- 71.
  15. Frayne, J. and C. R. Stokes. 1994. MHC class II positive cells and T cells in the equine endometrium throughout the oestrous cycle. Vet. Immunol. Immunopathol. 41:55-72. https://doi.org/10.1016/0165-2427(94)90057-4
  16. Barua, A., Y. Yoshimura and T. Tamura. 1998 a. The effects of age and sex steroids on the macrophage population in the ovary of the chicken, Gallus domesticus. J. Reprod. Fertil. 114:253-258. https://doi.org/10.1530/jrf.0.1140253
  17. Barua, A., Y. Yoshimura and T. Tamura. 1998b. Localization of macrophages in the ovarian follicles during the follicular growth and postovulatory regression in chicken, Gallus domesticus. Poult. Sci. 77:1417-1421. https://doi.org/10.1093/ps/77.9.1417
  18. Barua, A. and Y. Yoshimura. 1999 a. Immunolocalization of MHCII+ cells in the ovary of immature, young laying and old laying hens, Gallus domesticus. J. Reprod. Fertil. 116:385-389. https://doi.org/10.1530/jrf.0.1160385
  19. Barua, A., H. Michiue and Y. Yoshimura. 2001. Changes in the localization of MHC class II positive cells in hen ovarian follicles during the processes of follicular growth, postovulatory regression and atresia. Reprod. 121:953-957. https://doi.org/10.1530/rep.0.1210953
  20. Beckers, J. F., P. Ballman, F. Ectors, J. Derivaux and M. Herlant. 1975. Radioimmunoassay of bovine serum progesterone. CR Acad. Sci. Hedb. Seances Acad. Sci. D. 280:335-338.
  21. Begovich, A. B., T. L. Bugawan, B. S. Nepom, W. Klitz, G. T. Nepom and H. A. Erlich. 1989. A specific HLA-DP beta allele is associated with pauciarticular juvenile rheumatoid arthritis but not adult rheumatoid arthritis. Proc. Natl. Acad. Sci. USA, 86:9489-9493. https://doi.org/10.1073/pnas.86.23.9489
  22. Bissumbhar, B., P. R. Nilsson, E. J. Hensen, W. C. Davis and I. Joosten. 1994. Biochemical characterization of bovine MHC DQ allelic variants by one-dimensional isoelectric focusing. Tissue Antigens. 44:100-109. https://doi.org/10.1111/j.1399-0039.1994.tb02365.x
  23. Bugawan, T. L., G. Angelini, J. Larrick, S. Auricchio, G. B. Ferrara and H. A. Erlich. 1989. A combination of a particular HLA-DP beta allele and an HLA-DQ heterodimer confers susceptibility to coeliac disease. Nature. 339:470-473. https://doi.org/10.1038/339470a0
  24. Kaeoket, K., A. M., Dalin, U. Magnusson and E. Persson. 2001. The sow endometrium at different stages of the oestrous cycle: studies on the distribution of CD2, CD4, CD8 and MHC class II expressing cells. Anim. Reprod. Sci. 68:99-109. https://doi.org/10.1016/S0378-4320(01)00142-7
  25. Kaushic, C., E. Frauendorf, R. M. Rossoll, J. M. Richardson and C. R. Wira. 1998. Influence of the estrous cycle on the presence and distribution of immune cells in the rat reproductive tract. Am. J. Reprod. Immunol. 39:209-216. https://doi.org/10.1111/j.1600-0897.1998.tb00355.x
  26. Lewin, H. A., C. C. Calvert and D. Bernoco. 1985. Crossreactivity of a monoclonal antibody with bovine, equine, ovine, and porcine peripheral blood B lymphocytes. Am. J. Vet. Res. 46:785-788.
  27. Martinez, C. M., A. J. Buendia, J. Sanchez and J. A. Navarro. 2005. Immunophenotypical characterization of lymphocyte subpopulations of the uterus of non-pregnant and pregnant goats. Anat. Histol. Embryol. 34:240-246. https://doi.org/10.1111/j.1439-0264.2005.00606.x
  28. Morris, H., J. Edwards, A. Tiltman and M. Emms. 1985. Endometrial lymphoid tissue: an immunohistological study. J. Clin. Pathol. 38;644-652. https://doi.org/10.1136/jcp.38.6.644
  29. Palta, P., N. Bansal, R. S. Manik, B. S. Prakash and M. L. Madan. 1998. Interrelationships between follicular size, estradiol-17${\beta}$ (beta), progesterone and testosterone concentrations in individual buffalo ovarian follicles. Asian-Aust. J. Anim. Sci. 11:293-300. https://doi.org/10.5713/ajas.1998.293
  30. Wira, C. R. and R. M. Rossoll. 1995 a. Antigen-presenting cells in the female reproductive tract: influence of the estrous cycle on antigen presentation by uterine epithelial and stromal cells. Endocrinol. 136:4526-4534. https://doi.org/10.1210/en.136.10.4526
  31. Wira, C. R. and R. M. Rossoll. 1995b. Antigen-presenting cells in the female reproductive tract: influence of sex hormones on antigen presentation in the vagina. Immunol. 84:505-508.
  32. Wira, C. R. and R. M. Rossoll. 2003. Oestradiol regulation of antigen presentation by uterine stromal cells: role of transforming growth factor-beta production by epithelial cells in mediating antigen-presenting cell function. Immunol. 109:398-406. https://doi.org/10.1046/j.1365-2567.2003.01670.x
  33. Wira, C. R. and D. A. Sullivan. 1985. Estradiol and progesterone regulation of immunoglobulin A and G and secretory component in cervicovaginal secretions of the rat. Biol. Reprod. 32:90-95. https://doi.org/10.1095/biolreprod32.1.90
  34. Yoshimura, Y. 2004. Significance of local immunity in hen reproductive organs. Anim. Sci. J. 75:183-191. https://doi.org/10.1111/j.1740-0929.2004.00174.x
  35. Yu, M., R. P. Kinkel, B. Weinstock-Guttman, D. J. Cook and V. K. Tuohy. 1998. HLA-DP: a class II restriction molecule involved in epitope spreading during the development of multiple sclerosis. Hum. Immunol. 59:15- 24. https://doi.org/10.1016/S0198-8859(97)00252-8
  36. Rachman, F., V. Casimiri, A. Psychoyos and O. Bernard. 1983. Immunoglobulins in the mouse uterus during the oestrous cycle. J. Reprod. Fertil. 69:17-21. https://doi.org/10.1530/jrf.0.0690017
  37. Richeldi, L., R. Sorrentino and C. Saltini. 1993. HLA-DPB1 glutamate 69: a genetic marker of beryllium disease. Sci. 262: 242-244. https://doi.org/10.1126/science.8105536
  38. Tabibzadeh, S. S., S. Mortillo and M. A. Gerber. 1987. Immunoultrastructural localization of Ia antigens in human endometrium. Arch. Pathol. Lab. Med. 111:32-37.
  39. Tunon, A. M., H. Rodriguez-Martinez, A. Nummijarvi and U. Magnusson. 1999. Influence of age and parity on the distribution of cells expressing major histocompatibility complex class II, CD4, or CD8 molecules in the endometrium of mares during estrus. Am. J. Vet. Res. 60:1531-1535.
  40. Wallace, P. K., G. R. Yeaman, K. Johnson, J. E. Collins, P. M. Guyre and C. R. Wira. 2001. MHC class II expression and antigen presentation by human endometrial cells. J. Steroid. Biochem. Mol. Biol. 76:203-211. https://doi.org/10.1016/S0960-0760(00)00149-7
  41. Watson, E. D. 1985. Opsonising ability of bovine uterine secretions during the oestrous cycle. Vet. Rec. 117:274-275. https://doi.org/10.1136/vr.117.11.274
  42. Watson, E. D. and C. E. Dixon. 1993. An immunohistological study of MHC class II expression and T lymphocytes in the endometrium of the mare. Equine Vet. J. 25:120-124. https://doi.org/10.1111/j.2042-3306.1993.tb02920.x
  43. Watson, E. D. and S. R. Thomson. 1996. Lymphocyte subsets in the endometrium of genitally normal mares and mares susceptible to endometritis. Equine Vet. J. 28:106-110. https://doi.org/10.1111/j.2042-3306.1996.tb01600.x
  44. Zheng, W. M., M. Nishibori, N. Isobe and Y. Yoshimura. 2001. An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC Class II mRNA in the chicken oviduct. Reprod. 122:581-586. https://doi.org/10.1530/rep.0.1220581
  45. Zheng, W. M., Y. Yoshimura and T. Tamura. 1998. Effects of age and gonadal steroids on the localization of antigen-presenting cells, and T and B cells in the chicken oviduct. J. Reprod. Fertil. 114:45-54. https://doi.org/10.1530/jrf.0.1140045
  46. Eren, U., R. N. Astı, N. Kurtdede, M. Sandikcı and ve E. Sur. 1999. lnek uterusunda mast hucrelerinin histolojik ve histokimyasal ozellikleri ve mast hucre heterojenitesi. Turk. J. Vet. Anim. Sci. 23:193-201.
  47. Janzer-Pfeil, A. M. and G. A. Splitter. 1989. Identification of two independent MHC class II antigens in a bovine lymphoblastoid cell line. Vet. Immunol. Immunopathol. 22:307-319. https://doi.org/10.1016/0165-2427(89)90167-0
  48. Low, B. G., P. J. Hansen, M. Drost and K. J. Gogolin-Ewens. 1990. Expression of major histocompatibility complex antigens on the bovine placenta. J. Reprod. Fertil. 90:235-243. https://doi.org/10.1530/jrf.0.0900235
  49. Potolicchio, I., G. Mosconi, A. Forni, B. Nemery, P. Seghizzi and R. Sorrentino. 1997. Susceptibility to hard metal lung disease is strongly associated with the presence of glutamate 69 in HLA-DP beta chain. Eur. J. Immunol. 27:2741-2743. https://doi.org/10.1002/eji.1830271039
  50. Yoshimura, Y., T. Okamoto and T. Tamura. 1997. Localisation of MHC Class II, lymphocytes and immunoglobulins in the oviduct of laying and moulting hens. Br. Poult. Sci. 38:590- 596. https://doi.org/10.1080/00071669708418041

Cited by

  1. MHC Class II+ (HLA-DP-like) Cells in the Cow Reproductive Tract: II. Immunolocalization of MHC Class II+ Cells in Oviduct and Vagina vol.38, pp.4, 2009, https://doi.org/10.1111/j.1439-0264.2009.00940.x
  2. MHC class II expression by mast cells in the genital tract of cows vol.34, pp.5, 2010, https://doi.org/10.1007/s11259-010-9411-4
  3. Tissue distribution of some immune cells in bovine reproductive tract during follicular and luteal phase vol.81, pp.3, 2018, https://doi.org/10.1002/jemt.22983