Cloning, Expression, and Regulation of Bovine Cellular Retinoic Acid-binding Protein-II (CRABP-II) during Adipogenesis

  • Jeong, Young Hee ;
  • Lee, Sang Mi ;
  • Kim, Hye-Min ;
  • Park, Hyo Young ;
  • Yoon, Duhak ;
  • Moon, Seung Ju ;
  • Hosoda, Akemi ;
  • Kim, Dong-Ho ;
  • Saeki, Shigeru ;
  • Kang, Man-Jong
  • Received : 2007.09.18
  • Accepted : 2008.02.15
  • Published : 2008.11.01


The mammalian cellular retinoic acid-binding proteins, CRABP-I and CRABP-II, bind retinoic acid which acts as an inducer of differentiation in several biological systems. To investigate a possible role for CRABP-II in bovine adipogenesis, we have cloned bovine CRABP-II cDNA and the coding region for CRABP-I. The predicted amino acid sequences of CRABP-II were highly conserved among several animal species (human, mouse, and rat at 97%, 93%, and 93%, respectively). The expression pattern of bovine CRABP-II was examined in greater details by applying RT-PCR to various bovine tissues. CRABP-II mRNA was expressed in most adipose-containing tissues. Moreover, the expression of CRABP-I and -II mRNA dramatically increased during the differentiation of adipocytes from bovine intramuscular fibroblast-like cells. The effects of retinoic acid on adipocyte differentiation of bovine intramuscular fibroblast-like cells were concentration-dependent. Retinoic acid activated the formation of lipid droplets at a level of 1 nM, whereas inhibition was observed at a level of $1{\mu}M$. CRABP-I gene was up-regulated and CRABP-II gene down-regulated by retinoic acid during adipocyte differentiation. These results suggest that CRABPs may play an important role in the regulation of intracellular retinoic acid concentrations during adipogenesis.


Bovine Adipocyte Differentiation;Lipid-binding Protein;Retinoic Acid;Retinoic Acid Receptor;Retinoid X Receptor


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