The Effect of Dietary Supplementation of Fe-methionine Chelate and FeSO4 on the Iron Content of Broiler Meat

  • Seo, S.H. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Lee, H.K. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Ahn, H.J. (Cargill Korea Ltd.) ;
  • Paik, I.K. (Department of Animal Science & Technology, Chung-Ang University)
  • Received : 2007.03.16
  • Accepted : 2007.10.11
  • Published : 2008.01.01


A broiler experiment was conducted to compare the effects of supplementary iron sources and levels on the iron content of broiler meat. Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each treatment had 5 replicates of 10 birds (5 males and 5 females). Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control, Fe-Met 100 (100 ppm iron as Fe-methionine), Fe-Met 200, $FeSO_4$ 100 (100 ppm iron as $FeSO_4{\cdot}7H_2O$) and $FeSO_4\;200$. There were no significant differences among treatments in parameters related to production performance. Liver contained approximately 10 times more iron than the leg muscle which contained approximately 3 times more iron than either breast muscle or wing muscle. Significant differences in iron content in the broiler meat were observed. In the breast meat, Fe-Met treatments were significantly (p<0.05) higher than other treatments in iron content. In the leg meat, Fe-Met treatments and $FeSO_4\;200$ treatment were significantly higher than the control in iron content. In the wing muscle, Fe-Met 200 treatment was significantly higher than other treatments in iron content. Iron content in the liver was significantly influenced by source and supplementation level of iron. Fe-Met treatments were higher than $FeSO_4$ treatments and 200 ppm treatments were higher than 100 ppm treatments in iron content in the liver. It is concluded that iron-methionine chelate is more efficient than iron sulfate and 200 ppm iron supplementation as Fe-Met is recommended for maximum iron enrichment in broiler meat.


Supported by : Cargill Korea Inc.


  1. AOAC. 1990. Official Method of Analysis, 15th ed. Association of Official Analysis Chemists. Arlington, Virginia, USA.
  2. Aoyagi, S. and D. H. Baker. 1993. Protective effect of copperamino acid complexes against inhibitory effects of L-cysteine and ascorbic acid. Poult. Sci. 72(Suppl. 1):82(Abstr.).
  3. Ashmead, H. D. 1993. The role of amino acids chelates in animal nutrition. Noyes publications. New Jersey.
  4. Fouad, M. T. 1976. The physiochemical role of chelated minerals in maintaining optical body biological functions. J. Appl. Nutr. 28:5.
  5. Kim, D. H., J. S. Um, S. H. Ahn and I. K. Paik. 1997. Studies on the development and utilization of copper chelate as a supplement to the pig diet. Kor. J. Nutr. Feed. 21(5):429-438.
  6. Kratzer, F. H. and P. Vohra. 1986. Chelaes in nutrition. CRC Press, Inc., Boca Raton, Florida.
  7. Lim, H. S. and I. K. Paik. 2003. Effects of supplementary mineral methionine chelates (Zn, Cu, Mn) on the performance and eggshell quality of laying hens. Asian-Aust. J. Anim. Sci. 16(12):1804-1808
  8. .Lim, H. S., I. K. Paik, T. I. Sohn and W. Y. Kim. 2006. Effects of supplementary copper chelates in the form of methionine, chitosan and yeast on the performance of broilers. Asian-Aust. J. Anim. Sci. 19(9):1322-1327.
  9. Lim, H. S. and I. K. Paik. 2006. Effects of Dietary Supplementation of Copper Chelates in the Form of Methionine, Chitosans and Yeast in Laying Hens. Asian-Aust. J. Anim. Sci. 19(8):1174-1178.
  10. McNaugton, J. L., Day, B. C. Dilworth and B. D. Lott. 1974. Iron and copper availability from various sources. Poult. Sci. 53: 1325-1330.
  11. Miller, D., J. H. Soares, Jr., P. Bauersfeld, Jr. and S. L. Cupett. 1972. Comparative selenium retention by chicks fed sodium selenit, selenomethionine, fish meal and fish solubles. Poult. Sci. 51:1669-1673.
  12. NRC. 1994. Nutrient Requirements of Poultry. National Academy Press, Washington, DC.
  13. Paik, I. K. 2001. Application of chelated minerals in animal production. Asian-Aust. J. Anim. Sci. 14(Special Issue):191- 198.
  14. Park, S. W., H. Namkung, H. J. Ahn and I. K. Paik. 2004. Production of iron enriched eggs of laying hens. Asian-Aust. J. Anim. Sci. 17(12):1725-1728.
  15. Park, S. W., H. Namkung, H. J. Ahn and I. K. Paik. 2005. Enrichment of Vitamins D3, K and Iron in Eggs of Laying Hens. Asian-Aust. J. Anim. Sci. 18(2):226-229.
  16. SAS. 1995. SAS User's Guide: Statistics. Statistical Analysis System Institute Inc., Cary, NC.
  17. Spears, J. W. 1992. The bioavailability of zinc, copper and manganese amino acid complexes and chelates. NFIA., Nutrition Institute.
  18. Wedekind, K. J., A. E. Hortin and D. H. Baker. 1992. Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. J. Anim. Sci. 70:178-187.
  19. Wei, K. Q., Z. R. Xu, X. G. Luo, L. L. Zeng, W. R. Chen and M. F. Timothy. 2005. Asian-Aust. J. Anim. Sci. 18(10):1485-1491.
  20. Zoubek, G. L., E. R. Peo, Jr., B. D. Moser, T. Stahly and P. J. Cunningham. 1975. Effects of source on copper uptake by swine. J. Anim. Sci. 40:880-884.
  21. Duncan, D. B. 1995. Multiple range and multiple F tests. Biometrics. 11:1-12.

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