Heat Stress Causes Aberrant DNA Methylation of H19 and lgf-2r in Mouse Blastocysts

  • Zhu, Jia-Qiao (Gansu Agricultural University) ;
  • Liu, Jing-He (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science) ;
  • Liang, Xing-Wei (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science) ;
  • Xu, Bao-Zeng (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science) ;
  • Hou, Yi (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science) ;
  • Zhao, Xing-Xu (Gansu Agricultural University) ;
  • Sun, Qing-Yuan (State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science)
  • Received : 2007.07.06
  • Accepted : 2007.10.29
  • Published : 2008.04.30


To gain a better understanding of the methylation imprinting changes associated with heat stress in early development, we used bisulfite sequencing and bisulfite restriction analysis to examine the DNA methylation status of imprinted genes in early embryos (blastocysts). The paternal imprinted genes, H19 and Igf-2r, had lower methylation levels in heat-stressed embryos than in control embryos, whereas the maternal imprinted genes, Peg3 and Peg1, had similar methylation pattern in heat-stressed embryos and in control embryos. Our results indicate that heat stress may induce aberrant methylation imprinting, which results in developmental failure of mouse embryos, and that the effects of heat shock on methylation imprinting may be gene-specific.


Heat Stress;Methylation Imprinting;Mouse Embryos


Supported by : National Natural Science Foundation of China, Chinese Academy of Sciences


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