Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes

  • Yoon, D. (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) ;
  • Kwon, Y.S. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, K.Y. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jung, W.Y. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Korea) ;
  • Sasazaki, S. (Graduate School of Agricultural Science, Kobe University) ;
  • Mannen, H. (Graduate School of Agricultural Science, Kobe University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Received : 2007.04.11
  • Accepted : 2007.07.22
  • Published : 2008.01.01


In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, MseI, HpyCH4III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (I) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.


Supported by : Korean Science & Engineering Foundation


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