Expressed Sequence Tag Analysis of Antarctic Hairgrass Deschampsia antarctica from King George Island, Antarctica

  • Lee, Hyoungseok (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI)) ;
  • Cho, Hyun Hee (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI)) ;
  • Kim, Il-Chan (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI)) ;
  • Yim, Joung Han (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI)) ;
  • Lee, Hong Kum (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI)) ;
  • Lee, Yoo Kyung (Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI))
  • Received : 2007.09.19
  • Accepted : 2007.10.30
  • Published : 2008.04.30

Abstract

Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.

Keywords

Abiotic Stress;Antarctic;Deschampsia antarcitca;Expressed Sequence Tags (ESTs);King George Island;Quantitative Real Time Reverse Transcription PCR (qRT-PCR)

Acknowledgement

Supported by : Korea Research Foundation, Korea Polar Research Institute

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