Expression of Murine Asb-9 During Mouse Spermatogenesis

  • Lee, Man Ryul (Department of Anatomy and Cell Biology and Department of Biomedical Science, Hanyang University, College of Medicine) ;
  • Kim, Soo Kyoung (Department of Anatomy and Cell Biology and Department of Biomedical Science, Hanyang University, College of Medicine) ;
  • Kim, Jong Soo (Department of Anatomy and Cell Biology and Department of Biomedical Science, Hanyang University, College of Medicine) ;
  • Rhim, Si Youn (Department of surgery, Hanyang University, College of Medicine) ;
  • Kim, Kye-Seong (Department of Anatomy and Cell Biology and Department of Biomedical Science, Hanyang University, College of Medicine)
  • Received : 2008.07.07
  • Accepted : 2008.09.03
  • Published : 2008.12.31

Abstract

We previously showed that Asb-4 and Asb-17 is uniquely expressed in developing male germ cells. A recent report showed that Asb-9 is specifically expressed in the kidney and testes; however, detailed expression patterns in developing germ cells have not been shown. Northern blot analysis in various tissues demonstrated that mAsb-9 was strongly expressed in the testes. Expression analysis by RT-PCR and Northern blot in developing mouse testes indicates that mAsb-9 is expressed from the fourth week after birth to adulthood, with the highest expression in round spermatids. Expression sites were further localized by in situ hybridization in the testes. Pachytene spermatocytes and spermatids expressed mAsb-9 but spermatogonia and generated spermatozoa did not. This study reveals that mAsb-9 could be a specific marker of active spermatogenesis and would be useful for studies of male germ cell development.

Keywords

ankyrin repeat;Asb-9;SOCS box;spermatogenesis

Acknowledgement

Supported by : Korea Science and Engineering Foundation

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