Curcumin Inhibits Osteoclastogenesis by Decreasing Receptor Activator of Nuclear Factor-κB Ligand (RANKL) in Bone Marrow Stromal Cells

  • Oh, Sora (Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan) ;
  • Kyung, Tae-Wook (Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan) ;
  • Choi, Hye-Seon (Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan)
  • Received : 2008.06.04
  • Accepted : 2008.07.28
  • Published : 2008.11.30

Abstract

Curcumin (diferuloylmethane), a pigment derived from turmeric, has anti-oxidant and anti-inflammatory activities. Accumulating evidence points to a biochemical link between increased oxidative stress and reduced bone density. Osteoclast formation was evaluated in co-cultures of bone marrow stromal cells (BMSC) and whole bone marrow cells (BMC). Expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) was analyzed at the mRNA and protein levels. Exposure to curcumin led to dose-dependent suppression of osteoclastogenesis in the co-culture system, and to reduced expression of RANKL in $IL-1{\alpha}$-stimulated BMSCs. Addition of RANKL abolished the inhibition of osteoclastogenesis by curcumin, whereas the addition of prostaglandin $E_2$ ($PGE_2$) did not. The decreased osteoclastogenesis induced by curcumin may reduce bone loss and be of potential benefit in preventing and/or attenuating osteoporosis.

Keywords

bone;bone marrow stromal cells;curcumin;osteoclastogenesis;receptor activator of nuclear factor-${\kappa}B$ ligand

Acknowledgement

Supported by : University of Ulsan

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