Mapping, Tissue Distribution and Polymorphism of Porcine Retinol Binding Protein Genes (RBP5 and RBP7)

  • Gong, W.H. ;
  • Tang, Z.L. ;
  • Han, J.L. ;
  • Yang, S.L. ;
  • Wang, H. ;
  • Li, Y. ;
  • Li, K.
  • Received : 2007.02.09
  • Accepted : 2007.06.11
  • Published : 2008.11.01


The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol binding protein genes 5 and 7 (RBP5 and RBP7) were assigned to porcine chromosomes 5 and 6, respectively. The complete coding sequences (CDS) of the RBP5 and RBP7 genes were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) method, and the deduced amino acid sequences of both genes were compared to human corresponding proteins. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph node, small intestine, liver, brain, kidney and fat) were examined. A total of nine single nucleotide polymorphisms (SNPs) were identified in two genes. Three of these SNPs were analyzed using the polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP) method in Laiwu, Wuzhishan, Guizhou, Bama, Tongcheng, Yorkshire and Landrace pig breeds. Association analysis of genotypes of these SNP loci with economic traits was done in our experimental populations. Significant associations of different genotypes of $RBP5-A/G^{63}$, $RBP5-A/G^{517}$ and $RPB5-T/C^{intron1-90}$ loci with traits including maximum carcass length (LM), minimum carcass length (LN), marbling score (MS), back fat thickness at shoulder (SBF), meat color score (MCS) and hematocrit (HCT) were detected. These SNPs may be useful as genetic markers in genetic improvement for porcine production.


Retinol Binding Protein;Complete Coding Sequence;Porcine, Reverse Transcriptase Polymerase Chain Reaction;Single Nucleotide Polymorphism;Genotype


  1. Budhu, A. S. and N. Noy. 2002. Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest. Mol. Cell Biol. 22:2632-2641.
  2. Chambon, P. 1996. A decade of molecular biology of retinoic acid receptors. Faseb. J. 10:940-954.
  3. Conforti, L., A. Tarlton, T. G. Mack, W. Mi, E. A. Buckmaster, D. Wagner, V. H. Perry and M. P. Coleman. 2000. A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration $(WId^{S})$ mouse. Proc. Natl. Acad. Sci. USA. 97:11377-11382.
  4. Cowan, S. W., M. E. Newcomer and T. A. Jones. 1993. Crystallographic studies on a family of cellular lipophilic transport proteins: Refinement of P2 myelin protein and the structure determination and refinement of cellular retinolbinding protein in complex with all-trans-retinol. J. Mol. Biol. 230:1225-1246.
  5. Folli, C., V. Calderone, S. Ottonello, A. Bolchi, G. Zanotti, M. Stoppini and R. Berni. 2001. Identification, retinoid binding, and x-ray analysis of a human retinol-binding protein. Proc. Natl. Acad. Sci. USA. 98:3710-3715.
  6. Folli, C., V. Calderone, I. Ramazzina, G. Zanotti and R. Berni. 2002. Ligand binding and structural analysis of a human putative cellular retinol-binding protein. J. Biol. Chem. 277:41970-41977.
  7. Goureau, A., M. Yerle, A. Schmitz, J. Riquet, D. Milan, P. Pinton, G. Frelat and J. Gellin. 1996. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36:252-262.
  8. Lee, S. S., Y. Chen, C. Moran, A. Stratil, G. Reiner, H. Bartenschlager, G. Moser and H. Geldermann. 2003. Linkage and QTL mapping for Sus scrofa chromosome 5. J. Anim. Breed. Genet. 120:38-44.
  9. Malek, M., J. C. Dekkers, H. K. Lee, T. J. Baas and M. F. Rothschild. 2001. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mamm. Genome 12:630-636.
  10. Tang, Z. L., Y. Li, P. Wan, X. P. Li, S. H. Zhao, B. Liu, B. Fan, M. J. Zhu, M. Yu and K. Li. 2007. LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs. Genome Biol. 8(6):R115.
  11. Wang, Y. F., J. Yang, M. Yu, B. Liu, B. Fan, M. J. Zhu, T. A. Xiong and K. Li. 2005. Polymorphism detection of porcine PSMC3, PSMC6 and PSMD3 genes and their association with partial growth, carcass traits, meat quality and immune traits. Can. J. Anim. Sci. 85:475-480.
  12. Wang, H., Z. Zhu, H. Wang, S. Yang, D. Mo and K. Li. 2006a. Characterization of different expression patterns of calsarcin-1 and calsarcin-2 in porcine muscle. Gene 374:104-111.
  13. Wang, H. L., H. Wang, Z. M. Zhu, C. F. Wang, M. J. Zhu, L. Mo de, S. L. Yang and K. Li. 2006b. Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Mol. Genet. Genomics 276:264-272.
  14. Yu, M., Y. Wang, M. F. W. Te Pas, M. Yerle, B. Liu, B. Fan, T. Xiong and K. Li. 2004. Investigation of the porcine PA28 activator ${\gamma}$-subunit (PSME3) genes: isolation, polymorphism and its chromosomal localization. J. Anim. Breed. Genet. 121:142-148.

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Supported by : National Natural Science Foundation