Probiotics in Drinking Water Alleviate Stress of Induced Molting in Feed-deprived Laying Hens

  • Khajali, Fariborz (Department of Animal Science, Shahrekord University) ;
  • Karimi, S. (Laboratory for Animal Nutrition, University of Ghent) ;
  • Qujeq, D. (Department of Biochemistry and Biophysics, Babol University for Medical Sciences)
  • Received : 2007.12.29
  • Accepted : 2008.03.04
  • Published : 2008.08.01


An experiment was conducted to evaluate the physiological changes of laying hens subjected to feed removal during induced molting while received probiotics in the drinking water. Post-molt performance and egg quality criteria were also studied. Ninety 78-week-old Hy-line W36 laying hens were divided into two treatment groups according to equal body weight and subjected to induced molting by continuous feed removal until around 30% BW reduction. The experiment lasted 12 wks consisting of 4-wk molting and 8-wk post-molt periods. Treatment 1 received no probiotics and was considered as the control. Treatment 2 was similar to the control except that hens received probiotics in the drinking water at 400 mg/L during feed deprivation. The results indicated that hens in both groups went out of production by Day 5. However, hens received probiotics reached 5 and 50% egg production sooner than the control (30 and 52 days vs. 31 and 54 days). Starvation during molting increased heterophil to lymphocyte (H/L) ratio, hematocrit and plasma T4 and $Na^+$ levels while plasma T3 and Cl- levels were decreased. Probiotics had no significant impact on BW reduction during molt. Post-molt egg production and egg mass were higher in hens which previously received probiotics, but these responses were not significant. However, feed conversion ratio was significantly better in hens which received probiotics. Hematocrit, plasma thyroid hormone concentrations (T3 and T4) and plasma $Na^+$, $K^+$ and Cl- levels during molting were not significantly influenced by supplementation of probiotics. However, H/L ratio showed a significant (p<0.05) reduction in birds which received probiotics suggesting beneficial effects of this product for feed-deprived laying hens. No significant difference was observed in post-molt egg quality criteria.


Supported by : Shahekord University


  1. Berry, W. D. 2003. The physiology of induced molting. Poult. Sci. 82:971-980.
  2. Davis, G. S., K. E. Anderson and A. S. Carroll. 2002. The effect of long-term caging and molt of Single White Leghorn hens on heterophil to lymphocyte ratios, corticostrone, and thyroid hormones. Poult. Sci. 79:514-518.
  3. Decuypere, E., P. Van As, S. Van der Geyten and V. M. Darras. 2005. Thyroid hormone availability and activity in avian species: A review. Domes. Anim. Endocrin. 29:63-77.
  4. Dibner, J. J., C. Knight, G. F. Yi and J. D. Richards. 2007. Gut development and health in the absence of antibiotic growth promoters. Asian-Aust. J. Anim. Sci. 20:1007-1014.
  5. Gross, W. B. and H. L. Siegel. 1983. Evaluation of heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27:972-979.
  6. Fuller, R. 2001. The chicken gut microflora and probiotic supplements: A review. J. Poult. Sci. 38:189-196.
  7. Hayirli, A., N. Esenbulla, M. Macit, M. A. Yolk and H. Karaca. 2005. Nutrition practice to alleviate the adverse effects of stress on laying performance, metabolic profile and egg quality in peak producing hens: II. The probiotic supplementation. Asian-Aust. J. Anim. Sci. 18:1752-1760.
  8. Holt, P. S. 2003. Molting and Salmonella enterica serovar enteritidis infection: the problem and some solutions. Poult. Sci. 82:1008-1010.
  9. Keshavarz, K. and F. W. Quimby. 2002. An investigation of different molting techniques with an emphasis on animal welfare. J. Appl. Poult. Res. 11:54-67.
  10. Khajali, F., A. K. Zamani Moghaddam and E. Asadi Khoshouie. 2007. Application of an early skip-a-day feed restriction on physiological parameters, carcass traits and development of ascites in male broilers reared under regular or cold temperatures at high altitude. Anim. Sci. J. 78:159-163.
  11. Khajali, F., E. Asadi Khoshouie and F. Rafiei. 2007. Application of short fasting methods to induce molting in commercial laying hens. Proceedings of 16th European Poultry Nutrition Conference, Strasbourg, France.
  12. McDaniel, B. A. and D. R. Aske. 2000. Egg prices, feed costs, and the decision to molt. Poult. Sci. 79:1243-1245.
  13. North, M. O. and D. D. Bell. 1990. Commercial chicken production management. 4th edition. Chapman & Hall, New York.
  14. Revolledo, L., A. J. P. Ferreria and G. C. Mead. 2006. Prospects in salmonella control: competetive exclusion, probiotics, and enhancement of avian intestinal immunity. J. Appl. Poult. Res. 15:341-351.
  15. Reyns, G. E., K. A. Janssens, J. Buyse, E. R. Kuhn and V. M. Darras. 2002. Changes in thyroid hormone levels in chicken liver during fasting and refeeding. Comp. Biochem. Phys. Part B, 132:239-245.
  16. Ruszler, P. L. 1998. Health and husbandry considerations of induced molting. Poult. Sci. 77:1789-1793.
  17. Yoruk, M. A., M. Gul, A. Hayirli and M. Macit. 2004. The effects of supplementation of Humate and probiotics on egg production and quality parameters during late laying period in hens. Poult. Sci. 83:84-88.
  18. Yu, B., J. R. Liu, M. Y. Chiou, Y. R. Hsu and P. W. S. Chiou. 2007. The effect of probiotic Lactobacillus reuteri Pg4 strain on intestinal characteristics and performance in broilers. Asian-Aust. J. Anim. Sci. 20:1243-1251.

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