A Rapid and Sensitive Screening System for Human Type I Collagen with the Aim of Discovering Potent Anti-Aging or Anti-Fibrotic Compounds

  • Hashem, Md. Abul (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Jun, Kyu-Yeon (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Lee, Eunyoung (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Lim, Soyun (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Park Choo, Hea-Young (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University) ;
  • Kwon, Youngjoo (College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha Womans University)
  • Received : 2008.07.30
  • Accepted : 2008.09.04
  • Published : 2008.12.31

Abstract

This study was undertaken with the aim of developing an easy and quick means of analyzing the effect of various compounds on the synthesis and secretion of human type I collagen at the protein level. A modification of the ELISA method was used on HFF-1 cells. For the proof of concept, we used thirteen compounds most of which are known to be antioxidants. Each compound was tested at concentrations of 0, 10 and $100{\mu}m$ on HFF-1 cells for 24 h. Thirteen sets of experiments for each compound were performed in ANOVA with three replicates. Duncan multiple range test (DMRT) was used to compare the mean values obtained from the treatment groups. From the results it was concluded that Vitamin C, undecylenic acid, conjugated linoleic acid, glycolic acid, and citric acid at $100{\mu}m$ concentration could be used for anti-wrinkling or protection from premature aging, which requires enhancement of collagen synthesis. Lactic acid, EGCG, resveratrol, and retinol that can inhibit collagen synthesis effectively in a dose-dependent manner may be used for anti-fibrosis treatment purposes.

Keywords

anti-fibrosis;anti-wrinkling;collagen biosynthesis;HFF-1 cell;human type I collagen screening system

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