Growth Retardation and Death of Rice Plants Irradiated with Carbon Ion Beams Is Preceded by Very Early Dose- and Time-dependent Gene Expression Changes

  • Rakwal, Randeep (Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Kimura, Shinzo (Hazard Assessment and Epidemiology Research Group, Japan National Institute of Occupational Safety and Health (Japan NIOSH)) ;
  • Shibato, Junko (Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST)) ;
  • Nojima, Kumie (Research Center for Charged Particle Therapy, National Institute of Radiological Sciences (NIRS)) ;
  • Kim, Yeon-Ki (Division of Bioscience and Bioinformatics, Myongji University, GreenGene BioTech Inc.) ;
  • Nahm, Baek Hie (Division of Bioscience and Bioinformatics, Myongji University, GreenGene BioTech Inc.) ;
  • Jwa, Nam-Soo (Department of Molecular Biology, College of Natural Science, Sejong University) ;
  • Endo, Satoru (Research Institute for Radiation Biology and Medicine, Hiroshima University) ;
  • Tanaka, Kenichi (Research Institute for Radiation Biology and Medicine, Hiroshima University) ;
  • Iwahashi, Hitoshi (Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST))
  • Received : 2007.07.13
  • Accepted : 2007.12.12
  • Published : 2008.04.30


The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.


DNA Microarray;Early Gene Expression;Radiation;Rice;Stress Response


Supported by : Atomic Energy Commission


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