Development of a Sequence Characteristic Amplified Region Marker linked to the L4 Locus Conferring Broad Spectrum Resistance to Tobamoviruses in Pepper Plants

  • Kim, Hyun Jung (Department of Plant Sciences, Seoul National University) ;
  • Han, Jung-Heon (Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Yoo, Jae Hyoung (Joongbu Breeding and Research Station, SeminisKorea) ;
  • Cho, Hwa Jin (Joongbu Breeding and Research Station, SeminisKorea) ;
  • Kim, Byung-Dong (Department of Plant Sciences, Seoul National University)
  • Received : 2007.07.05
  • Accepted : 2007.12.03
  • Published : 2008.04.30

Abstract

To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

Keywords

Amplified Fragment Length Polymorphism(AFLP);Bulked Segregant Analysis (BSA);Capsicum;L4;Marker Conversion;Sequence Characterized Amplified Region (SCAR);Tobamoviruses

Acknowledgement

Supported by : Korea Science and Engineering Foundation (KOSEF)

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