Stage-specific Expression of Ankyrin and SOCS Box Protein-4 (Asb-4) during Spermatogenesis

  • Kim, Soo-Kyoung (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Rhim, Si Youn (Department of Surgery, Hanyang University) ;
  • Lee, Man Ryul (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Kim, Jong Soo (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Kim, Hyung Jun (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University) ;
  • Lee, Dong Ryul (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University) ;
  • Kim, Kye-Seong (Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University)
  • Received : 2007.11.05
  • Accepted : 2007.12.13
  • Published : 2008.04.30

Abstract

Members of the large family of Asb proteins are ubiquitously expressed in mammalian tissues; however, the roles of individual Asb and their function in the developmental testes have not been reported. In this report, we isolated a murine Asb4 from mouse testis. Northern blot analysis revealed that mAsb-4 was expressed only in testes and produced in a stage-specific manner during spermatogenesis. It was expressed in murine testes beginning in the fourth week after birth and extending into adulthood. Pachytene spermatocytes had the highest level of expression. Interestingly, the human homologue of mAsb-4, ASB-4 (hASB-4) was also expressed in human testis. These results suggest that ASB-4 plays pivotal roles in mammalian testis development and spermatogenesis.

Keywords

Ankyrin-Repeat;Asb-4;SOCS Box;Spermatogenesis

Acknowledgement

Supported by : Hanyang University

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