Marker-Assisted Foreground and Background Selection of Near Isogenic Lines for Bacterial Leaf Pustule Resistant Gene in Soybean

  • Kim, Kil-Hyun (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Moon-Young (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Van, Kyu-Jung (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Moon, Jung-Kyung (Department of Functional Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Dong-Hyun (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Lee, Suk-Ha (Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Published : 2008.12.31

Abstract

Bacterial leaf pustule (BLP) caused by Xanthomonas axonopodis pv. glycines is a serious disease to make pustule and chlorotic haloes in soybean [Glycine max (L). Merr.]. While inheritance mode and map positions of the BLP resistance gene, rxp are known, no sequence information of the gene was reported. In this study, we made five near isogenic lines (NILs) from separate backcrosses (BCs) of BLP-susceptible Hwangkeumkong $\times$ BLP-resistant SS2-2 (HS) and BLP-susceptible Taekwangkong$\times$ SS2-2 (TS) through foreground and background selection based on the four-stage selection strategy. First, 15 BC individuals were selected through foreground selection using the simple sequence repeat (SSR) markers Satt486 and Satt372 flanking the rxp gene. Among them, 11 BC plants showed the BLP-resistant response. The HS and TS lines chosen in foreground selection were again screened by background selection using 118 and 90 SSR markers across all chromosomes, respectively. Eventually, five individuals showing greater than 90% recurrent parent genome content were selected in both HS and TS lines. These NILs will be a unique biological material to characterize the rxp gene.