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Anti-cancer Activity of Flavonoids from Aceriphyllum rossii

돌단풍(Aceriphyllum rossii)에서 분리한 플라보노이드의 항암활성

  • Ahn, Eun-Mi (Department of Herbal Foodceutical Science, Daegu Haany University) ;
  • Han, Jae-Taek (Chemport Inc.) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Sung-Hoon (College of Oriental Medicine, KyungHee University) ;
  • Baek, Nam-In (Institute of Life Science & Resources, and Plant Metabolism Research Center, KyungHee University)
  • 안은미 (대구한의대학교 한방식품약리학과) ;
  • 한재택 ((주)켐포트) ;
  • 권병목 (한국생명공학연구원) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 백남인 (경희대학교 생명자원과학연구원 및 식물대사연구센터)
  • Published : 2008.12.31

Abstract

The methanol extract from the aerial parts of Aceriphyllum rossii was fractionated into ethyl acetate, n-BuOH and $H_2O$ layers through solvent fractionation. Repeated silica gel column chromatography of EtOAc and n-BuOH layers afforded five flavonol glycosides. They were identified as astragalin (1), kaempferol 3-O-${\alpha}$-L-rhamnopyranosyl (1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (2), rutin (3), kaempferol 3-O-${\alpha}$-L-rhamnopyranosyl (1${\rightarrow}$4)-${\alpha}$-L-rhamnopyranosyl 1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (4), and quercetin 3-O-${\alpha}$-L-rhamnopyranosyl (1${\rightarrow}$4)-${\alpha}$-L-rhamnopyranosyl (1${\rightarrow}$6)-${\beta}$-D-glucopyranoside (5) on the basis of spectroscopic data. All of them showed an inhibition in farnesyl protein tranferase (FPTase) activity, and rutin (3) inhibited the growth of rat H-ras cell and the cell migration of human umbilical vein endothelial cells (HUVECs).

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