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Anti-stress Effect during Long and Short-Term of Vitamin E in Mice

마우스에서 vitamin E의 장.단기적 항스트레스 효과

  • Lee, Seung-Jin (Department of Bioengineering at the Postgraduate School, Konkuk University) ;
  • Go, Min-Seok (Department of Bioengineering at the Postgraduate School, Konkuk University) ;
  • Kang, Sang-Mo (Department of Bioengineering at the Postgraduate School, Konkuk University)
  • 이승진 (건국대학교 대학원 생물공학과) ;
  • 고민석 (건국대학교 대학원 생물공학과) ;
  • 강상모 (건국대학교 대학원 생물공학과)
  • Published : 2008.12.31

Abstract

This study was to evaluate the protective effect of vitamin E against long and short-term stress in ICR mice. Two groups which had been bred for 5 months (equivalent to human beings aged 20) were treated by immobilization stress for 8 weeks with or without vitamin E, and one out of two groups was continuously bred until they become 18 months old (equivalent to human senescence) with or without Vitamin E. Afterwards, the changes of serum and hepatic metabolites were investigated on the basis of the index of stress-related in vivo oxidative damage. As a result, it was found that stress increases serum triacylglycerol and aspartate aminotransferase (AST) in the long and short-term, and decreases serum HDL-cholesterol. In addition, stress concerned the decrease of total antioxidant status (TAS) and superoxide dismutase (SOD) as well as the increase of malondialdehyde (MDA) in liver. These results suggest that stress in one’s youth causes negative results in TG, HDL-cholesterol, AST, TAS, SOD and MDA measured in one’s senescent. The administration of vitamin E in the stressed mice decreases serum TG and AST that are increased by stress, and exerts influence on the increase of serum HDL-cholesterol. Also vitamin E recovered the values of liver TAS, SOD and MDA in the stressed mice. In conclusion, vitamin E represented protective effect in the stressed mice to some degree.

References

  1. Chow, C. K. (1991) Vitamin E and oxidative stress. Free Radical Bio. Med. 11, 215-232 https://doi.org/10.1016/0891-5849(91)90174-2
  2. Tiidus, P. M. and Houston, M. E. (1995) Vitamin E status and response to exercise training. Sports Med. 20, 12-23 https://doi.org/10.2165/00007256-199520010-00002
  3. Collett, C., Pares-Herbute, N., Monnier, L. H. and Cartry, E. (1988) Platelet function in type I diabetes: Effects of supplementation with large doses of vitamin E. Am. J. Clin. Nutr. 47, 256-261 https://doi.org/10.1093/ajcn/47.2.256
  4. Lovin, R., Cottle, W., Pyke, I., Davanagh, M. and Belcastro, A. N. (1987) Are induces of free radical damage related to exercise intensity. Eur. J. Appl. Physiol. 6, 313-316
  5. Lawler, J. M., Powers, S. K., Dijk, H. V., Visser, T., Kordus, M. J. and Ji, L. L. (1994) Metabolic and antioxidant enzyme activities in the diaphragm: effects of acute exercise. Res. Physiol. Neurobiol. 96, 139-149
  6. Yun Mei, K. R., Gawai., Zhongzhen, Nie., Vickmar. and Robert, H. Helfert. (1999) Age related reductions in the activities of antioxidant enzymes in the rat inferior colliculus. Hearing. Res. 135, 169-180 https://doi.org/10.1016/S0378-5955(99)00103-3
  7. Okimoto, K., Hamazaki, K., Lwagaki, H., Orita, K. and Mori, A. (1995) Effect of picibanil on the scavenging effect of free radicals produced during liver regeneration in the rat. Acta. Med. Okayama. 49, 75-79
  8. Bedossa, P., Houglum, K., Trautwein, C., Holstege, A. and Chojkier, M. (1994) Stimulation of collagen $\alpha$ I(I) gene espression is associated with lipid peroxidation in hepatocellular injury: a Link tot issue fibrosis. Hepatology. 19, 1262-1271
  9. Armendariz-Borunda, J., Seyer, J. M., Kang, A. H. and Raghow, R. (1990) Regulation of TGF-$\beta$ gene espression in rat liver intoxicated with carbon tetrachloride. FASEB J. 4, 215-221 https://doi.org/10.1096/fasebj.4.2.2298342
  10. Castilla, A., Prieto, J. and Fausto, N. (1991) Transforming growth factor $\beta$1 and $\alpha$ in chronic liver disease. N. Eng. J. Med. 324, 933-940 https://doi.org/10.1056/NEJM199104043241401
  11. Scarpa, M., Rigo, A., Maiorino, M., Ursini, F. and Gregolin, C. (1984) Formation of $\alpha$-tocopherol radical and recycling of $\alpha$- tocopherol by ascorbate during peroxidation of phophatidylcholin liposomes. An electron paramagnetic resorbance study. Biochem. Biophys. Res. Comm. 801, 215-219
  12. Elliot, G. R. and Eisdorfer, C. (1982) Stress and human health. New York, Springer, 2, 1-20
  13. Brenner, D. A., Veloz, L., Jaenisch, R. and Alcorn, J. M. (1993) Stimulation of the collagen $\alpha$ I (I) endogenous gene and transgene in carbon tetrachloride-induced hepatic fibrosis. Hepatology. 17, 287-292
  14. Hernry, J. B. (2007) Measurement of lipids and evaluation of lipid disorders. In Clinical diagnosis and management by Laboratory Methods, Levy, M. D. pp. 171-198, Elsevier Science Health Science div, Churchill
  15. Halliwell, B. and Gutteridge, J. M. C. (1989) In Free radicals in biology and medicine, (2nd ed.) Clarendon Press, Oxford
  16. Park, S. Y. (2001) Study on the oxidative damage induced by stress and its recovery in mice. Department of Microbial Engineering. Dissertation of Ph. D. Konkuk Universtiy. Seoul
  17. Rao, M. S., Pareddy, K., Abecassis, M. and Hashimoto, T. (2001) Regeneration of liver with marked fatty change following partial hepatectomy in rats. Digest. Dis. Sci. 46, 1821-1826 https://doi.org/10.1023/A:1010654908938
  18. Livne, E., Laufer, D. and Blumenfeld, I. (1997) Comparison of in vitro response to growth hormone by chondrocytes from mandibular condyle cartilage of young and old mice. Calcified. Tissue. Int. 7, 61-62
  19. Kruit, J. K., Groen, A. K., van Berkel, T. J. and Kuipers, F. (2006) Emerging roles of the intestine in control of cholesterol metabolism. World. J. Gastroenterol. 12, 6429-6439 https://doi.org/10.3748/wjg.v12.i40.6429
  20. Cherubini, A., Zuliani, G. and Costantini, F. (2001) High vitamin E plasma levels and low low-density lipoprotein oxidation are associated with the absence of atheros-clerosis in octogenarians. J. Am. Geriatr. Soc. 49, 651-654 https://doi.org/10.1046/j.1532-5415.2001.49128.x
  21. Traber, M. G. (2001) Does vitamin E decrease heart attack risk summary and implications with respect to dietary recommendations. J. Nutr. 131, 395-397 https://doi.org/10.1093/jn/131.2.395S
  22. Gittleman, B., Shatin, L., Bierenbaum, M. L., Fleisohman, A. I. and Hqyton, T. (1968) Effects of quantified stressful stimuli on blood lipids in man. J. Nutr. Ment. Dis. 147, 196-201 https://doi.org/10.1097/00005053-196808000-00011
  23. Woo, Y. S. (1992) Studies on the effects of slaughter stress on the blood picture and serum chemical values of pigs. Dissertation of Ph. D. Konkuk Universtiy. Seoul
  24. Thomson, C. B. (1995) Apoptosis in the pathogenesis and treatment of disease. Science. 267, 1456-1462 https://doi.org/10.1126/science.7878464
  25. Kissebah, A. H. (1974) Stress hormines and lipid metabolism. Proc. R. Soc. Med. 67, 665-667
  26. Howard, N. H., Dieter, M. K., Poetro, A., Gabriele, B. B., Giuseppe, G., Juliana, H., Hazel, P. and Alex, S. (1994) Biochemical and cytotoxic characterstics of an in vivo circulating oxidized LDL. J. Lipid Res. 35, 669-677
  27. Lim, S. S., Kim, M. H. and Lee, J. H. (1997) Effect of Artemisia princeps var orientalis and Circium japonicum var ussuriense on liver function body lipid and bile acid of hyperlipidemic rat. Korean J. Nutr. Soc.. 30, 797-802
  28. Nakatsukasa, H., Evarts, R. P., Hsia, C. C. and Thorgeirsson, S. S. (1990) Transforming growth factor-$\beta^1$1 and type I procollagen transcripts during regeneration and early fibrosis of rat liver. Lab Invest. 63, 171-180
  29. Lehtonen, A. J. (1978) The effect of vigorous physical activity at work on serum lipids with a special reference to serum highdensity lipoprotein cholesterol. Acta. Physiol. Scand. 104, 117-121 https://doi.org/10.1111/j.1748-1716.1978.tb06257.x
  30. Avellini, L., Chiaradia, E. and Gaiti, A. (1999) Effect of exercise training, selenium and vitamin E on some free radical sacvengers in horse. Comp. Biochem. Phys. B. 123, 147-154 https://doi.org/10.1016/S0305-0491(99)00045-0
  31. Ji, L. L. (1993) Blood glutathione status during exercise; Effect of carbohydrate supplementation. J. Appl. Physiol. 74, 788-792. 26 https://doi.org/10.1152/jappl.1993.74.2.788
  32. Gutteridge, J. N. C. (1995) Lipid peroxidation and antioxidants as biomarker of tissue damages. Clin. Chem. 41, 1819-1828
  33. Kagan, V. E., Spirichev, V. B. and Serbinova, E. A. (1994) The significance of vitamin E and free radicals in physical exercise. In Nutrition in exercise and sport, Wolinsky, I. (2nd ed.) pp. 185-213, CRC Press, Boca Raton
  34. Gale, C. R., Ashurst, H. E., Powers, H. J. and Martyn, C. N. (2001) Antioxidant vitamin status and carotid atherosclerosis in the elderly. Am. J. Clin. Nutr. 74, 402-408 https://doi.org/10.1093/ajcn/74.3.402
  35. Ghrousos, G. P. (2000) The role of stress and the hypothalamicpituitary-adrenal axis in the pathogenesis in the metabolic syndrome: Neuroendocrine and target tissue-related causes. Int. J. Obesity. 24, S50-S55 https://doi.org/10.1038/sj/ijo/0801278

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