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Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas

  • Ji, Xiuhong (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Huh, Bum (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Soo-Un (Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University)
  • Published : 2008.12.31

Abstract

To establish the biosynthetic pathway of decursin in Angelica gigas Nakai, feeding experiment with stable isotope-labeled precursors were conducted. Umbelliferone and decursin were labeled with deuterium at C-3. The umbelliferone, the decursin, and other commercially available putative precursors, L-phenylalanine-ring-$d_5$ and trans-cinnamic acid-$d_7$, were fed to the hairy root culture of A. gigas. Each deuterated compound was incorporated into decursinol, decursinol angelate, and decursin as determined by mass spectrometric analysis. These findings confirmed the coumarin biosynthesis pathway sequence is composed of phenylalanine, cinnamic acid, umbelliferone, decursinol, and decursin.

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Cited by

  1. Current status on the development of molecular markers for differentiation of the origin of Angelica spp. vol.44, pp.1, 2017, https://doi.org/10.5010/JPB.2017.44.1.012
  2. Natural Korean Medicine Dang-Gui: Biosynthesis, Effective Extraction and Formulations of Major Active Pyranocoumarins, Their Molecular Action Mechanism in Cancer, and Other Biological Activities vol.22, pp.12, 2017, https://doi.org/10.3390/molecules22122170