DOI QR코드

DOI QR Code

Degradation of Cyanide by Activated Sludge Immobilized with Polyethylene Glycol

고정화 활성슬러지를 이용한 시안 분해

  • Cheong, Kyung-Hoon (Department of Environmental Engineering and BK21 Team for Biohydrogen Production, Chosun University) ;
  • Choi, Hyung-Il (Department of Environmental Engineering, Chosun University) ;
  • Kim, Jung-Ae (Department of Environmental Engineering, Chosun University) ;
  • Moon, Ok-Ran (Department of Environmental Engineering, Chosun University) ;
  • Kim, Myung-Hee (Department of Chemistry, Chonnam University)
  • 정경훈 (조선대학교 환경공학과 BK21 바이오 가스기반 수소생산 사업팀) ;
  • 최형일 (조선대학교 환경공학과) ;
  • 김정애 (조선대학교 환경공학과) ;
  • 문옥란 (조선대학교 환경공학과) ;
  • 김명희 (전남대학교 화학과)
  • Published : 2008.12.31

Abstract

The activated sludge obtained from wastewater coke oven plant was immobilized by entrapment with polyethylene glycol (PEG). The effects of several factors on the biodegradation of $CN^-$ from. synthetic wastewater were investigated using batch and continuous reactors. The degradation rate of $CN^-$ increased with increasing of the immobilized bead volume in the reactor. Approximately 7.65mg/L of $NH_4-N$ was produced upon the degradation of 35mg/L of $CN^-$. When high concentrations of the toxic cyanide complex were used in the testing of cyanide degradation, the free activated sludge could be inhibited more than that of the immobilized activated sludge. When the phenol concentration was higher than 400mg/L in the synthetic wastewater, approximately 98.4% of $CN^-$ was removed within 42 hours by the immobilized activated sludge. However, the cyanide was not completely degraded by the tree activated sludge. This indicates that high phenol concentrations can act as a toxic factor for the free activated sludge. A $CN^-$ concentration of less than 1mg/L was achieved by the immobilized sludge at the loading rate of 0.025kg $CN^-/m^3-d$. Moreover, it was found that the HRT should be kept for 48 hours in order to obtain stable treatment conditions.

Keywords

Immobilization;Activated sludge;Cyanide degradation;Polyethylene glycol

References

  1. Kjeldsen P., 1999, Behaviour of cyanide in soil and groundwater, A review, Water Air Soil Poll., 115, 279-307 https://doi.org/10.1023/A:1005145324157
  2. Yanase H., Sakamoto A., Okamoto K., Kita K., Sato Y., 2000, Degradation of the metal-cyano complex tetracyanonicke1ate(II) by Fusarium oxysporium N-10, Appl. Microbiol. Biotechnol., 53, 328-334 https://doi.org/10.1007/s002530050029
  3. Figueria M. M., Ciminelli V. S. T., De Andrade M. C., Linardi V. R., 1996, Cyanide degradation by an Escherichia coli strain, Can. J. Microbiol., 42, 519-523 https://doi.org/10.1139/m96-070
  4. Kao C. M., Lin C. C., Liu J. K., Chen Y. L., Wu L. T., Chen S. C., 2004, Biodegradation of the metal-cyano complex tetracyanonickelate( II) by Klebsiella oxytoca, Enzyme Microb. Technol., 35, 405-410 https://doi.org/10.1016/j.enzmictec.2004.05.010
  5. Dursan A. T., Tepe O., 2005, Internal mass transfer effect on biodegradation of phenol by Ca-alginate immobilized Ralstonia eutropha, J. Hazard. Mater., B126. 105-111
  6. Kwon H. K., 2002, Biological treatment and degradation metabolism of cyanide compounds by microorganisms isolated from wastewater of coke oven plant, ph. D. Dissertation, Dept. of Chemical Engineering, Pohang University of Science and Technology, Pohang Korea
  7. 橋本 奬, 古川 憲治, 濱 宏, 1985, 活性汚泥の 固定 化と その淨化機能に 關す る 硏究, 下水道協會誌, 22, 42-45
  8. 김선일, 윤영재, 정경훈, 1996, 광경화성 수지에 고정화된 활성슬러지에 의한 페놀 분해, 한국생물공학회지, 11(5), 577-585
  9. Dursan A. T., Akuz Z., 2000, Biodegradation kinetics of ferrous(II) cyanide complex ions by immobilized Pseudomonas fluorecens in packed bed column reactor, Process Biochem, 35, 615-622 https://doi.org/10.1016/S0032-9592(99)00110-7
  10. Sumino T., Nakamura H., Yukio K., 1992, Immobilization of Nitrifying bacteria by polyethylene glycol prepolymer, J. Ferment. Bioeng., 73, 37-42 https://doi.org/10.1016/0922-338X(92)90228-M
  11. 김종택, 1999, 수질오염공정시험법 해설, 신광출판사, 174-282pp
  12. Kowalska M., Bodzek M., Bohdziewicz J., 1998, Biodegradation of phenol and cyanide using membrane with immobilized microorganisms, Process Biochemistry, 33(2), 189-197 https://doi.org/10.1016/S0032-9592(97)00103-9
  13. Kunz D. A., Nagappan O., Silva-avalos J., Delong G., 1992, Utilization of cyanide as a nitrogenous substrate by Pseudomonas fluorescens NCIM B 11764, Evidence for multiple pathways of metabolic conversion, App. Environ. Microbiol., 58, 2022-2029
  14. Tanaka H., Harada S., Kurosawa H., 1987, A new immobilized cells system with protection against toxic solvent, Biotechnology and Bioengineering, 30, 22-30 https://doi.org/10.1002/bit.260300105
  15. Knowles C. J., Bunch A. W., 1986, Microbial cyanide metabolism, Advances in Microbial Physiology. 27, 73-111 https://doi.org/10.1016/S0065-2911(08)60304-5
  16. 박영규, 이철희, 박수정, 1995, 포괄고정화법에 의한 PVA 함유폐수의 처리특성, 대한환경공학회지, 16(8), 985-993
  17. Jeong Y. S., Chung J. S., 2006, Simultaneous removal of COD, thiocyanate, cyanide and nitrogen from coal process wastewater using fluidized biofilm process, Process Biochem., 41, 1141-1147 https://doi.org/10.1016/j.procbio.2005.12.010
  18. Kang S. M., Kim J. M., 1993, Degradation of cyanide by a bacterial mixture composed of new types of cyanide-degrading bacteria, Biotech. Lett., 15, 201-206 https://doi.org/10.1007/BF00133024
  19. Shivaraman N., Parhad N. M., 1985, Biodegradation of cyanide by Pseudomonas acidovorans and influence of pH and phenol, Indian Journal of Microbiology. 25, 79-82, 1985
  20. Watanabe A., Yano K., Ikebukuro K., Karube I., 1998, Cyanide hydrolysis in a cyanide-degrading bacterium, Pseudomonas stutzeri AK61 by cyanidase, Microbiology, 144, 1677-1682 https://doi.org/10.1099/00221287-144-6-1677
  21. Gijzen H. J., Bernal E., Ferrer H., 2000, cyanide toxicity and cyanide degradation in anaerobic wastewater treatment, Water Res., 34, 2447-2454 https://doi.org/10.1016/S0043-1354(99)00418-2
  22. Sirianuntapiboon S., Chuamkaew C., 2007, Packed cage rotating biological contactor system for treatment of cyanide wastewater, Bioresource Technol., 98, 266-272 https://doi.org/10.1016/j.biortech.2006.01.014
  23. Campos M. G., Pereira P., Roseiro J. C., 2006, Packed-bed reactor for the integrated biodegradation of cyanide and formamide by immobilized Fusarium oxysporum CCMI 876 and Methylobacterium sp. RXM CCMI 908, Enzyme and Microbial Technology, 38, 848-854 https://doi.org/10.1016/j.enzmictec.2005.08.008
  24. Dias J. C. T., Rezende R. P., Linardi V. R., 2001, Bioconversion of nitriles by Candida guilliermondii CCT 7207 cells immobilized in barium alginate, Appl. Microbiol. Biotechnol., 56, 757-761 https://doi.org/10.1007/s002530100681
  25. Chen C. Y., Kao C. M., Chen S. C., 2008, Application of Klebsiella oxytoca immobilized cells on the treatment of cyanide wastewater, Chemosphere, 71, 133-139 https://doi.org/10.1016/j.chemosphere.2007.10.058