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Electron Microscopic Study for the Influence of Soaking in Hot Water and Prochloraz Solution on Spore and Mycelium of Fusarium fujikuroi Infected in Rice Seed

온탕소독과 prochloraz 침지소독이 벼 종자에 감염된 Fusarium fujikuroi의 포자와 균사의 형태에 미치는 영향에 대한 전자현미경적 연구

  • Park, Woo-Sik (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Yeh, Wan-Hae (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Se-Weon (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Han, Seong-Suk (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lee, Jun-Seong (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science) ;
  • Lim, Chun-Keun (Division of Bio-Resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Yong-Hwan (Agricultural Microbiology Division, Department of Agricultural Biology, National Academy of Agricultural Science)
  • 박우식 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 예완해 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 한성숙 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이준성 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 임춘근 (강원대학교 농업생명과학대학 응용생물공학과) ;
  • 이용환 (농촌진흥청 국립농업과학원 농업미생물과)
  • Published : 2008.12.01

Abstract

This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

Keywords

Hot water;Prochloraz;SEM;Seed disinfection

References

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  3. Large-scale screening of rice accessions to evaluate resistance to bakanae disease vol.80, pp.5, 2014, https://doi.org/10.1007/s10327-014-0528-0
  4. Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease vol.17, pp.3, 2011, https://doi.org/10.5423/RPD.2011.17.3.288