Effect of Chlorella vulgaris on lipid metabolism in Wistar rats fed high fat diet

  • Lee, Hee-Sun (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Park, Hoon-Jung (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Kim, Mi-Kyung (Department of Nutritional Science and Food Management, Ewha Womans University)
  • Published : 2008.12.31


This study was performed to investigate effects of Chiarella vulgaris on lipid metabolism in rats fed high fat diet. Sixty 6-week-old male Wistar rats were divided into two groups; normal diet group and high fat diet group, then the rats in each group were further divided into three subgroups and fed 0%, 5% and 10% (w/w) chlorella-containing diets, respectively, and raised for 9 weeks, Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and total protein and albumin concentration were not different among groups. Serum total lipids and liver TG concentration were significantly lower in 5% and 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Serum TG, serum total cholesterol, liver total lipid and liver total cholesterol concentrations were significantly lower in 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Fecal total lipid, TG and total cholesterol excretions were significantly higher in 5% and 10% chlorella groups than 0% chlorella groups in normal diet and high fat diet groups, respectively (p<0.05). These results suggest that Chlorella vulgaris is effective for prevention of dyslipidemia which may be due to the modulation of lipid metabolism and increased fecal excretion of lipid.


  1. Bligh EG & Dyer WJ (1959). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917 https://doi.org/10.1139/o59-099
  2. Boden G, Chen X & Iqbal N (1998). Acute lowering of plasma fatty acids lowers basal insulin secretion in diabetic and nondiabetic subjects. Diabetes 47:1609-1612 https://doi.org/10.2337/diabetes.47.10.1609
  3. Chau CF & Huang YL (2005). Effects of the insoluble fiber derived from Passiflora edulis seed on plasma and hepatic lipids and fecal output. Mol Nutr Food Res 49:786-790 https://doi.org/10.1002/mnfr.200500060
  4. Cherng JY & Shih MF (2005a). Potential hypoglycemic effects of Chlorella in streptozo-tocin induced diabetic mice. Life Sci 77:980-990 https://doi.org/10.1016/j.lfs.2004.12.036
  5. Cherng JY & Shih MF (2005b). Preventing dyslipidemia by Chlorella pyrenoidosa in rats and hamsters after chronic high fat diet treatment. Life Sci 76:3001-3013 https://doi.org/10.1016/j.lfs.2004.10.055
  6. Cherng JY & Shih MF (2006). Improving glycogenesis in Streptozocin (STZ) diabetic mice after administration of green algae Chlorella. Life Sci 78:1181-1186 https://doi.org/10.1016/j.lfs.2005.06.050
  7. Frings CS & Dunn RT (1970). A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am J Clin Pathol 53:89-91 https://doi.org/10.1093/ajcp/53.1.89
  8. Ghosh P, Bitsanis D, Ghebremeskel K, Crawford MA & Poston L (2001). Abnormal aortic fatty acid composition and small artery function in offspring of rats fed a high fat diet in pregnancy. J Physiol 533:815-822 https://doi.org/10.1111/j.1469-7793.2001.00815.x
  9. Han JG, Kang GG, Kim JK & Kim SH (2002). The present status and future of chlorella. Food Science and Industry 6:64-69
  10. Huff MW & Carroll KK (1980). Effects of dietary protein on turnover, oxidation and absorption of cholesterol, and on steroid excretion in rabbits. J Lipid Res 21:546-558
  11. Iwami K, Sakakibara K & Ibuki F (1986). Involvement of postdigestion hydrophobic peptides in plasma cholesterol-lowering effect of dietary plant protein. Agric Biol Chem 50:1217-1222 https://doi.org/10.1271/bbb1961.50.1217
  12. Kang MS, Sim AJ & Chae HJ (2004). Chlorella as a Functional Biomaterial. Korean Journal of Biotechnology and Bioengineering 19:1-11
  13. Kay PA (1991). Microalgae as food and supplement. Crit Rev Food Sci Nutr 30:555-573 https://doi.org/10.1080/10408399109527556
  14. Konishi F, Mitsuyama M, Okuda M, Tanaka K, Hasegawa H & Nomoto K (1996). Protective effect of an acidic glycoprotein obtained from culture of Chlorella vulgaris against myelosuppression by 5-fluorouracil. Cancer Immunol Immunother 42:268-274 https://doi.org/10.1007/s002620050281
  15. Kupeli E, Orhan DD & Yesilada E (2006). Effect of Cistus laurifolius L. leaf extracts and flavonoids on acetaminophen induced hepatotoxicity in mice. J Ethnopharmacol 103:455-460 https://doi.org/10.1016/j.jep.2005.08.038
  16. Layne E (1957). Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3:447-454 https://doi.org/10.1016/S0076-6879(57)03413-8
  17. Lee HS, Choi CY, Cho C & Song Y (2003). Attenuating Effect of Chlorella Supplementation on Oxidative Stress and NF.KAPPA.B Activation in Peritoneal Macrophages and Liver of C57BL/6 Mice Fed on an Atherogenic Diet. Biosci Biotechnol Biochem 67:2083-2090 https://doi.org/10.1271/bbb.67.2083
  18. Lee SO, Simons AL, Murphy PA & Hendrich S (2005). Soyasaponins lowered plasma cholesterol and increased fecal bile acids in female golden Syrian hamsters. Exp Biol Med 230:472-478 https://doi.org/10.1177/153537020523000705
  19. Nagata Y, Ishiwaki N & Sugano M (1982). Studies on the mechanism of antihypercholesterolemic action of soy protein and soy proteintype amino acid mixtures in relation to the casein counterparts in rats. J Nutr Biochem 112:1614-1625
  20. Okudo M, Hasegawa T, Sonoda M, Okabe T & Tanaka M (1975). The effects of Chlorella on the level of cholesterol in serum and liver. Jap J Nutrition 33:3-8 https://doi.org/10.5264/eiyogakuzashi.33.3
  21. Park HS, Park JY & Cho HJ (2005). Attitudes and reported practice for obesity management in Korea after introduction of anti-obesity agents. J Korean Med Sci 20:1-6 https://doi.org/10.3346/jkms.2005.20.1.1
  22. Queiroz ML, Bincoletto C, Valadares MC, Dantas DC & Santos LM (2002). Effects of Chlorella vulgaris extract on cytokines production in Listeria monocytogenes infected mice. Immunopharmacol Immunotoxicol 24:483-496 https://doi.org/10.1081/IPH-120014731
  23. Reaven GM, Chang H, Ho H, Jeng CY & Hoffman BB (1988). Lowering of plasma glucose in diabetic rats by antilipolytic agents. Am J Physiol 254:E23-E30
  24. Reeves PG, Nielsen FH & Fahey GC Jr (1993). AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939-1951 https://doi.org/10.1093/jn/123.11.1939
  25. Reitman A & Frankel S (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56-63 https://doi.org/10.1093/ajcp/28.1.56
  26. Rizvi F, Iftikhar M & George JP (2003). Beneficial effects of fish liver preparations of sea bass (Lates calcarifer) versus gemfibrozil in high fat diet-induced lipid-intolerant rats. J Med Food 6:123-128 https://doi.org/10.1089/109662003322233521
  27. Roden M, Price TB, Perseghin G, Petersen KF, Rothman DL, Cline GW & Shulman GI (1996). Mechanism of free fatty acid-induced insulin resistance in humans. J Clin Invest 97:2859-2865 https://doi.org/10.1172/JCI118742
  28. Saloranta C, Franssila-Kallunki A, Ekstrand A, Taskinen MR & Groop L (1991). Modulation of hepatic glucose production by nonesterified fatty acids in type 2 (non-insulin-dependent) diabetes mellitus. Diabetologia 34:409-415 https://doi.org/10.1007/BF00403179
  29. Sano T & Tanaka Y (1987). Effects of dried powdered Chlorella vulgaris on experimental atherosclerosis and alimentary hypercholesterolemia in cholesterol-fed rabbit. Artery 14:760-784
  30. Sano T, Kumamoto Y, Kamiya N, Okuda M & Tanaka Y (1988). Effect of lipophilic extract of Chlorella vulgaris on alimentary hyperlipidemia in cholesterol-fed rats. Artery 15:217-224
  31. Shibata S, Oda K, Onodera-Masuoka N, Matsubara S, Kikuchi- Hayakawa H, Ishikawa F, Iwabuchi A & Sansawa H (2001). Hypocholesterolemic effect of indigestible fraction of Chlorella regularis in cholesterol-fed rats. J Nutr Sci Vitaminol 47:373-377 https://doi.org/10.3177/jnsv.47.373
  32. Singh A, Singh SP & Bamazai R (1998). Perinatal influence of Chlorella vulgaris on hepatic drug metabolizing enzyme and lipis. Anticancer Res 18:1509-1514
  33. Smith C, Marks AD & Lieberman M (2005). Basic medical biochemistry, p.478-479. Lippincott Williams & Wilkins, Baltimore. USA
  34. Steinberg D (1991). Antioxidants and atherosclerosis. A current assessment 84:1240-1245 https://doi.org/10.1161/01.CIR.84.3.1420
  35. Storlien LH, Baur LA, Kriketos AD, Pan DA, Cooney GJ, Jenkins AB, Calvert GD & Campbell LV (1996). Dietary fats and insulin action. Diabetologia 39:621-631 https://doi.org/10.1007/BF00418533
  36. Surwit RS, Kuhn, CM, Cochrane C, McCubbin, JA & Feinglos MN (1988). Diet-induced type II diabetes in C57BL/6J mice. Diabetes 37:1163-1167 https://doi.org/10.2337/diabetes.37.9.1163
  37. Tanaka K, Yamada A, Nada K, Shoyama Y, Kubo C & Nomoto K (1997). Oral administration of a unicellular green algae, Chlorella vulgaris, prevents stress-induced ulcer. Plant Med 63:465-466 https://doi.org/10.1055/s-2006-957736
  38. Tanaka K, Yamada A, Noda K, Hasegawa T, Okuda K, Shoyama Y & Nomoto K (1998). A novel glycoprotein obtained from Chlorella vulgaris strain CK22 shows antimetastatic immunopotentiation. Cancer Immunol Immunother 45:313-320 https://doi.org/10.1007/s002620050448
  39. The Koreas Society of Food Science and Nutrition (2000). Handbook of experimental in food science and nutrition, p.661. Hyoil Press, Seoul. Republic of Korea