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Characterization of Bacillus thuringiensis subsp. tohokuensis CAB167 Isolate against Mosquito Larva

모기유충에 활성 있는 Bacillus thuringiensis subsp. tohokuensis CAB167 균주의 특성

  • Kil, Mi-Ra (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Da-A (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Paek, Seung-Kyoung (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Kim, Jin-Su (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Choi, Su-Yeon (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Jin, Da-Yong (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Youn, Young-Nam (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University) ;
  • Hwang, In-Chon (Central Research Institute, Kyung Nong Co.) ;
  • Ohba, Michio (Bioresources and Management Laboratory, Graduate School of Agriculture, Kyushu University) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Science, Chungnam National University)
  • 길미라 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김다아 (충남대학교 농업생명과학대학 응용생물과) ;
  • 백승경 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김진수 (충남대학교 농업생명과학대학 응용생물과) ;
  • 최수연 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김대용 (충남대학교 농업생명과학대학 응용생물과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물과) ;
  • 황인천 ((주) 경농 중앙연구소) ;
  • ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물과)
  • Published : 2008.12.30

Abstract

Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. $LC_{50}$ values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE. Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.

References

  1. Cannon, R.J.C. 1996. Bacillus thuringiensis use in agriculture: A molecular perspective. Biol. Rev. Cambridge Phil. Soc. 71: 561-636 https://doi.org/10.1111/j.1469-185X.1996.tb01285.x
  2. Ishii. T and M. Ohba. 1994. The 23-kilodalton CytB protein is solely responsible for mosquito larvicidal activity of Bacillus thuringiensis serovar kyushuensis. Curr. Microbiol. 29: 91-94 https://doi.org/10.1007/BF01575754
  3. Kim, H.S., H.W. Park, D.W. Lee, Y.M. Yu, J.I. Kim and S.K. Kang. 1995a. Distribution and characterization of Bacillus thuringiensis isolated from soils in Korea. Kor. J. Appl. Entomol. 34(4): 344-349
  4. Klowden, M.J., G.A. Held and L.A.B. JR. 1983. Toxicity of Bacillus thuringiensis subsp. israelensis to adult Ades aegypti Mosquitoes. Appl. Environ. Microbiol. 312-315
  5. Koni, P.A and D.J .Ellar. 1993. Cloning and characterizaion of a novel Bacillus thuringiensis cytolytic delta-endotoxin. J. Mol. Biol. 229: 319-327 https://doi.org/10.1006/jmbi.1993.1037
  6. Mizuki, E., M. Ohba, T. Akao, S. Yamashita, H. Satio and Y.S. Park. 1999. Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cell. Appl. Microbiolo. 86: 477-486
  7. Ohba, M., H. Iwahana. and S. Asano. 1992. Unique isolate of Bacillus thuringiensis serovar japonensis with a high larvicidal activity specified for scarabaeid beetles. Lett. Appl. Microbiol. 14: 54-57 https://doi.org/10.1111/j.1472-765X.1992.tb00646.x
  8. Zouari, Nabil and J. Samir. 1997. Purification and immunological characterization of particular delta-endotoxins from three strains of Bacillus thuringiensis. Biotechol. Lett. 19(8): 825-829 https://doi.org/10.1023/A:1018364915612
  9. Chilcott, C.N and P.J. Wigley. 1994. Opportunities for finding new Bacillus thuringiensis sratin collection. Appl. Eviron. Microbiolo. 64:4965-4972
  10. Ohba, M and K. Aizawa. 1990. Occurrence of two pathotypes in Bacillus thuringiensis subsp. fukukaensis (Flagella Serotype 3a: 3d: 3e). J. Invertebr. Pathol. 59: 99-103 https://doi.org/10.1016/0022-2011(92)90118-N
  11. Khodyrev, V.P., G.V. Kalmykova, L.I. Burtseva and V.V. Glupov. 2006. Characterizaion of crystal-forming bacteria Bacillus thuringiensis subsp. tohokuensis toxic to mosquite larvae. Biolo. Bull. 33(5): 513-516 https://doi.org/10.1134/S1062359006050141
  12. Federici, B.A. 1993. Insecticidal bacterial proteins identify the midgut epithelium as a source of novel target site for insect control. Arch. Insect. Biochem. Physiol. 22: 357-371
  13. Ohba, M., K. Aizawa and S. Shimizu. 1981. A new subspecies of Bacillus thuringiensis isolated in Japan: Bacillus thruingiensis subsp. tohokuensis (Serotype 17). J. Invertebr. Pathol. 38: 307 -309 https://doi.org/10.1016/0022-2011(81)90140-3
  14. Kim H.S., H.W. Park, D.W. Lee, Y.M. Yu and S.K. Kang. 1995c. Characterization of Bacillus thuringiensis isolated in Granary Dusts. Kor. J. Appl. Entomol. 34(3): 243-248
  15. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680 -685 https://doi.org/10.1038/227680a0
  16. WHO. 2005. Guidelines for lavoratory and field testing of mosquito larvicides. World Health Organizaion communicable disease control, prevention and eradication. WHO pesticide evaluation scheme
  17. Crickmore, N., Zeigler. D.R and J. Feitelson. 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. & Mol. Biolo. Rev. 62: 807-813
  18. Kim H.S., D.W. Lee, H.W. Park, Y.M. Yu, J.I. Kim and S.K. Kang. 1995b. Distribution and characterization of Bacillus thuringiensis isolated from soils of sericultural farms in Korea. Kor. J. Appl. Entomol. 37(1): 57-61
  19. Yu, Y. M., M. Ohba and S. S. Gill. 1991. Characterization of mosquitocidal activity of Bacillus thuringiensis subsp. fukuokaensis crystal proteins. Appl. Environ. Microbiol. 57(4): 1075-1081
  20. Choi S.Y., S.C. Oh, M.S. Cho, S.K. Paek, J.S. Kim, D.A. Kim, M.R. Gill, Y.N. Youn and Y.M. Yu. 2007. Bioassay of environment -friendly insecticides for management of mosquito, Culex pipiens molestus. Kor. J. Appl. Entomol. 46(2): 261-267 https://doi.org/10.5656/KSAE.2007.46.2.261
  21. Dulmage, H.T. 1970. Insecticidal activity of HD-1, a new isolate of Bacillus thuringiensis var. alesti. J. Inverte. Pathol. 15: 232 -239 https://doi.org/10.1016/0022-2011(70)90240-5
  22. Ohgushi, A., N. Wasano and N. Shisa. 2003. Characterizaion of mosquiocidal Bacillus thuringiensis serovar sotto strain isolated from Okinawa, Jap. J. Appl. Microbiol. 95: 982-989 https://doi.org/10.1046/j.1365-2672.2003.02068.x
  23. Padua, L.E., M. Ohba and K. Aizawai. 1984. Isolation of a Bacillus thruingiensis strian (Serotype 8a:8b) highly and selectively toxic against mosquito larvae. J. Invertebr. Pathol. 44: 12-17 https://doi.org/10.1016/0022-2011(84)90040-5
  24. Choi S.Y., M.S. Cho, T.H. Kim, J.S. Kim, S.K. Paek, D.A., Y.N. Youn, S.S. Hong and Y.M. Yu. 2008. Bioactive characterization of Bacillus thuringiensis subsp. kurstaki CAB133 isolated from domestic soil. Kor. J. Appl. Entomol. 47(2): 175-184 https://doi.org/10.5656/KSAE.2008.47.2.175
  25. Hernstadt, C., G.G. Soares, E.R. Wilcox and D.L. Edwards. 1986. A new strain of Bacillus thuringiensis with activity against coleoptetan insects. Biotechnol. 4: 305-308 https://doi.org/10.1038/nbt0486-305
  26. Yu, Y.M., M. Ohba and K. Aizawa. 1987. Synergistic effects of the 65- and 25-kilodalton proteins of Bacillus thuringiensis strain PG-14 (serotype 8a:8b) in mosquito larvicidal activity. J. Gen. Appl. Microbiol. 33: 459-462 https://doi.org/10.2323/jgam.33.459
  27. Ohgushi, A., H. Satioh, W. Naoya., A. Uemori and M. Ohba. 2005. Cloning and characterization of two novel genes, cry24B and s1orf2, from a mosquitocidal strain of Bacillus thuringiensis servar sotto. Curr. Microbiol. 51: 131-136 https://doi.org/10.1007/s00284-005-7529-3
  28. Ohba, M., H. Saitoh, K. Miyamoto, K. Higuchi. 1995. Bacillus thuringiensis serovar higo (flagellar serotype 44), a new serogroup with a larvicidal activity preferential for the anopheline mosquito. Letter in appl. Micro. 21(5): 316 https://doi.org/10.1111/j.1472-765X.1995.tb01068.x
  29. De Barros Moreira Beltrao, H and M.H. Silva-filha. 2007. Interaction of Bacillus thuringiensis svar. israelensis cry toxins with binding sites from Aedes aegyti (Diptera: Culicidae) larvae midgut. FEMS. Micro. Lett. 266(2): 163-169 https://doi.org/10.1111/j.1574-6968.2006.00527.x
  30. Benintende, G.B., J.E. Lopez-Meza, J.G. Cozzi, C.F. Piccinetti, and J.E. Ibarra. 2000. Characterizaion of INTA 51-3, a new atypical strain of Bacillus thuringiensis from Argentina. Curr. Microbiol. 41: 396-401 https://doi.org/10.1007/s002840010157
  31. Wasano, N., K.H. Kim, M. Ohba. 1998. Delta-endotoxin proteins associated with spherical parasporal inclusions of the four Lepidoptera -specific Bacillus thuringiensis strains. Appl. Micro. 84(4): 501 -508 https://doi.org/10.1046/j.1365-2672.1998.00371.x
  32. Nickerson, K.W. and L.A.Jr. Bulla. 1974. Physiology of sporeforming bacteria associated with insects minial nutritional requirement for growth sporulation and parasporal crystal formation in Bacillus thuringiensis. Appl. Environ. Microbiol. 28: 124-128
  33. Zhong, C., D.J. Ellar, A. Bishop, C. Johnson, S. Lin and E.R. Hart. 2000. Characterizaion of a Bacillus thuringiensis $\delta$-endotoxin which is toxic to insects in three orders. J. Invertebr. Pathol. 76: 131-139 https://doi.org/10.1006/jipa.2000.4962
  34. Federici, B.A., Luhy. P and J.E. Ibrarra. 1990. The parasporal body of Bacillus thuringiensis subsp. israelensis: structure, protein composition and toxicity. In: de Barjae, H and Sutherland, S., (eds) Bacterial control of mosquitoes and blackflies: Biochemistry, genetics and applications of Bacillus thuringiensis and Bacillus sphaericus. New Brunsick, NJ: Rutgers University Press. 16-24
  35. Glare, T.R. and M. O'Callaghan. 2000. Bacillus thuringiensis: Biology, ecology and safety. Chichester: Wiley. 350.pp
  36. Kim, D.A., J.S. Kim, M.R. Kil, Y.N. Youn, D.S. Park and Y.M. Yu. 2006. Isolation and activity of insect pathogenic Bacillus thuringiensis strain from soil. Kor. J. Appl. Entomol. 45(3): 357-362

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