Changes of Feeding Behaviors of Myzus persicae (Homoptera: Aphididae) Depending on Inflow Concentrations of Imidacloprid

  • Seo, Mi-Ja (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kang, Myong-Ki (Dongbu Hiteck) ;
  • Jo, Beom-Haeng (Central Research Institute) ;
  • Hwang, In-Cheon (Central Research Institute) ;
  • Jang, Chul (Central Research Institute) ;
  • Yu, Yong-Man (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young-Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Published : 2008.12.30


After treatment with imidacloprid, there were clear differences in the time to the first reaction of Myzus persicae among the concentrations treated. The time taken for the proboscis of the aphids to penetrate, during the recording plants increased as the imidacloprid concentration increased. Imidacloprid concentration inflow into a leaf was investigated using high-performance liquid chromatography, and the residues of the imidacloprid varied slightly with the different concentrations treated. However, the inflow rates of this insecticide into a leaf increased as the dipping times increased. Furthermore, it was shown that there was no relationship in inflow concentration between the concentrations and times of treatment. However, the concentration in the leaf differed according to the dipping time. Judging from the fact that the first reaction behavior against imidacloprid displayed at an inflow concentration of 0.32-0.35 mg/L, we concluded that inflow concentrations causing the first reaction of the aphids to the insecticide were much lower than the concentration treated. The general feeding characteristics of the aphids indicated that xylem and/or phloem feeding behavior continued after a series of probing behaviors and stylet activity during the first 3 h from the start of EPG recording. After 90 min treatment with imidacloprid, feeding behavior over the next 30 min indicated a significant increase in the withdrawal of the stylet from the plant at all treated concentrations. Xylem and/or phloem feeding patterns were significantly decreased during this time. In particular, the proportion of xylem feeding differed according to the concentration of imidacloprid.


  1. Nauen, R. 1995. behavior modifying effects of low systemic concentrations of imidacloprid on Myzus persicae with special reference to an antifeeding response. Pestic. Sci. 44: 145-153
  2. Prado, E. and W.F. Tjallingii. 1994. Aphid activities during sieve element punctures. Ent. Exp. Appl. 72: 157-165
  3. Sauge, M.H., J.P. Lacroze, J.L. Poessel, T. Pascal and J. Kervella. 2002. Induced resistance by Myzus persicae in the peach cultivar 'Rubira'. Ent. Exp. Appl. 102: 29-37
  4. Hashiba, T. and T. Misawa. 1969. Studies on the mechanism of aphid transmission of stylet-borne virus III. On the adherence of the virus to the stylet. Tohoku J. Agri. Res. 20: 159-171
  5. Mizell, R.F. and M.C. Sconyers. 1992. Toxicity of imidacloprid to selected arthropod predators in the laboratory. Florida Entomol. 75: 277-280
  6. Nauen, R., U. Ebbinghaus-Kintscher, A. Elbert, P. Jeschke, K. Tietjen. 2001. Acetylcholine receptors as sites for developing neonicotinoid insecticides. In: Ishaaya, I. (ed.), Biochemical Sites of Insecticides Action and Resistance. Springer, New York, pp. 77-105
  7. Lowery, D.T. and M.K. Sears. 1986. Stimulation of reproduction of the green peach aphid (Homoptera: Aphididae) by azinphosmethyl applied to potatoes. J. Econ. Entomol. 79: 1530-1533
  8. McLean, D.L. and M.G. Kinsey. 1964. A technique for electrically recording aphid feeding and salivation. Nature 202: 1358-1359
  9. Ponder, K.L., J. Pritchard, R. Harrington and J.S. Bale. 2000. Difficulties in location and acceptance of phloem sap combined with reduced concentration of phloem amino acids explain lowered performance of the aphid Rhopalosiphum padi on nitrogen deficient barley (Hordeum vulgare) seedlings. Ent. Exp. Appl. 97: 203-210
  10. Seo, M.J., J.K. Jang, E.J. Kang, M.K. Kang, N.S. Kim, Y.M. Yu, Y.N. Youn. 2005. Feeding behavior in the plant tissues with green peach aphid (Myzus persicae, Aphididae; Homoptera) using EPG technique. Kor. J. Appl. Entomol. 44: 271-276
  11. Youn, Y.N. M.J. Seo, J.G. Shin, C. Jang, and Y.M. Yu. 2003. Toxicity of greenhouse pesticides to multicolored Asian lady beetles, Harmonia axyridis (Coleoptera: Coccinellidae). Biol. Control 28: 164-170
  12. Harrewijn, P. and H. Kayser. 1997. Pymetrozine, a fast-acting and selective inhibitor of aphid feeding. In-situ studies with electronic monitoring of feeding behavior. Pestic. Sci. 49: 130-140<130::AID-PS509>3.0.CO;2-U
  13. Gorden, P.L. and F.L. McEwen. 1984. Insecticide-stimulated reproduction of Myzus persicae, the green peach aphid (Homoptera: Aphididae). Can. Entomol. 116: 783-784
  14. Tjallingii, W.F., 1988. Electrical recording of stylet penetration activities by aphids. In: Campbell, R.K. Eikenbary, R.D. (eds.), Aphid-Plant Genotype Interactions. Elsevier, Amsterdam. pp. 89-99
  15. Kim, S., S. Seo, J.D. Park. S.G. Kim,. and D.I. Kim. 2005. Effects of selected the predatory mite, Amblyseius cucumeris (Acari: Phytoseiidae). J. Entomol. Sci. 40: 107-114
  16. Leicht, W. 1993. Imidacloprid-a chloronicotinyl insecticide. Pest Outlook 4: 1724
  17. Youn, Y.N. 1998. Electrically recorded feeding behavior of Nephotettis cincticeps. J. Asia-Pac Entomol. 1: 147-161
  18. James, D.G. and B. Vogele. 2001. The effect of imidacloprid on survival of some beneficial arthropods. Plant Protect Quart 16: 58-62

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