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Adipocyte-Related Genes and Transcription Factors were Affected by siRNA for Aromatase Gene during 3T3-L1 Differentiation

지방세포 분화중인 3T3-L1 세포에서 아로마테이즈 siRNA 처리에 의한 지방관련 유전자와 전사인자의 발현 조절

  • Jeong, Dong-Kee (Department of Animal Biotechnology, College of Applied Biosciences, Cheju National University, The Institute of Subtropical Biological Science)
  • 정동기 (제주대학교 생명자원과학대학 생명공학부 동물생명공학, 아열대생명과학 연구소)
  • Published : 2008.11.30

Abstract

This study was performed to verify the gene expression of 3T3-L1 using the siRNA of the aromatase gene, which is the estrogen synthesis enzymes. First of all three pairs of siRNA were designed from the CYP19A1 (aromatase) and analyzed the formation of fat cell mechanism by transferring gene to 3T3-L1 and differentiating it. As a result, the expression of leptin gene, which is the main gene causing the obesity, was controlled and the cause of the obesity is related with the insulin specifically. The overexpression of adiponectin and adipsin was observed. This result showed that the formation of the fat was controlled a little without any side effect by obstructing a specific material out of all the signal systems in the fat formation. This study will be an important clue to make it clear that the lack or overexpression of estrogen might be the cause of fat formation mechanism.

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