Comparative Analysis of Dissimilatory Sulfite Reductase (dsr) Gene from Sediment of Lake Sihwa, Korea and Lake Aha, China

한국 시화호와 중국 Aha호 저질토에 분포하는 이화성 아황산염 환원효소 유전자의 비교 분석

  • Kim, In-Seon (Department of Environmental Science, Kangwon National University) ;
  • Kim, Ok-Sun (School of Biological Sciences, Seoul National University) ;
  • Jeon, Sun-Ok (Department of Environmental Science, Kangwon National University) ;
  • Witzel, Karl-Paul (Max Planck Institute of Evolutionary Biology) ;
  • Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University)
  • 김인선 (강원대학교 환경과학과) ;
  • 김옥선 (서울대학교 생명과학부) ;
  • 전선옥 (강원대학교 환경과학과) ;
  • ;
  • 안태석 (강원대학교 환경과학과)
  • Published : 2008.06.30

Abstract

The diversity of sulfate reducing bacteria was investigated in different depths of sediments in Lake Sihwa, Korea and Lake Aha, China by PCR amplification, denaturing gradient gel electrophoresis (DGGE) and clone libraries targeting dissimilatory sulfite redectase (dsr) gene. In the analysis of DGGE band patterns, the community compositions of dsr gene in the sediments of both lakes were significantly different whereas bands in all depths of each environment revealed similar patterns. Bands from Lake Sihwa were produced much more than those from Lake Aha, demonstrating a higher diversity of dsr gene in Lake Sihwa. Total 68 clones containing dsr gene were obtained to analyze their sequences. Sequences from the sediment of Lake Sihwa were affiliated to Deltaproteobacteria, the Gram-positive thermophilic sulfate reducers belonging to the genus Desulforomaculum and archaeal thermophilic SRB belonging to the genus Archaeoglobus, whereas sequences from the sediments of Lake Aha were related to genus Desulfotomaculum. Clones retrieved from sediment of Lake Sihwa revealed a higher numbers than those of Lake Aha, demonstrating a higher diversity of dsr gene in Lake Sihwa. Most of clones (59%) were distantly related to the known cultivated SRB with $60\sim65%$ of similarity, which were clustered only the sequences from the environments showed less than 90% similarity. These habitat specific sequences suggested that the clustered dsr sequences represent species or groups of species that were indigenous to these environments. This study showed that these lakes have a specific bacterial communities having dsr gene distinct from those in other environments such as soil and marine ecosystems around the world.

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