DOI QR코드

DOI QR Code

Composition of Phytoplankton in Gamak Bay by CHEMTAX Analyses

CHEMTAX 활용한 가막만 식물플랑크톤 군집조성

  • Oh, Hyun-Taik (National Fisheries R&D Institute, Marine Environmental Management Division) ;
  • Kim, Da-Jung (National Fisheries R&D Institute, Marine Environmental Management Division) ;
  • Lee, Won-Chan (National Fisheries R&D Institute, Marine Environmental Management Division) ;
  • Jung, Rae-Hong (National Fisheries R&D Institute, Marine Environmental Management Division) ;
  • Hong, Suk-Jin (National Fisheries R&D Institute, Marine Environmental Management Division) ;
  • Kang, Yang-Sun (NFRDI, Marine Ecology Division) ;
  • Lee, Yang-Woo (Busan National Univ. Dept. Biology) ;
  • Tilburg, Charles (Univ. New England, Dept. Chemistry & Physics)
  • 오현택 (국립수산과학원 환경관리과) ;
  • 김다정 (국립수산과학원 환경관리과) ;
  • 이원찬 (국립수산과학원 환경관리과) ;
  • 정래홍 (국립수산과학원 환경관리과) ;
  • 홍석진 (국립수산과학원 환경관리과) ;
  • 강양순 (국립수산과학원 해양생태과) ;
  • 이용우 (부산대학교 생물학과) ;
  • Published : 2008.10.30

Abstract

Chlorophyll a (chl a) has been used as an indicator for phytoplankton biomass in pelagic ecosystems due to the relative ease of measurement and selectivity for autotrophs in mixed plankton assemblages. However, the use of chi a as an indicator for phytoplankton biomass is restricted due to its inability to resolve taxonomic differences of phytoplankton and the highly variable relationship of chi a with phytoplankton. Here, we describe the analysis of High-Performance Liquid Chromatography (HPLC) photosynthetic pigment data using CHEMTAX, which is a matrix factorization program that uses chemical taxonomic indices (phytoplankton carotenoids) to quantify the abundance of phytoplankton groups. Compared to direct microscopic counting that can distinguish species within broad groups, the resolution of taxonomic groups by CHEMTAX is generally coarse. It can only distinguish between diatoms, dinoflagellates, cryptophytes, cyanobacteria, chlorophytes, prasinophytes, and haptophytes. However, CHEMTAX analysis is much faster and less expensive than microscopic counting methods. HPLC pigment observations were taken in the spring, summer, fall, and winter in$ 2005\sim2006$ within Gamak Bay, South Korea. CHEMTAX results revealed that diatoms were the dominant taxonomic group in Gamak Bay. In inner Gamak Bay, the ratio between diatoms and cryptophytes was $75\sim80%$, and the ratio between dinoflagellates and cryptophytes was $10\sim15%$. In outer Gamak Bay, the ratio between diatoms and cryptophytes was $85\sim90%$, and the ratio between dinflagellates and cryptophytes was only $1\sim5%$. The population structure was seasonal. Relative diatom populations were less in the summer than the winter season.

Keywords

Gamak Bay;Pigments;HPLC;CHEMTAX

References

  1. Jeffrey S. W., Mantoura R. F., Wright S. W., 1997, Phytoplankton pigments in oceanography: Guideline to modern methods, UNESCO Publishing, 74-75
  2. Forsberg G., Ryding S. O., 1980, Eutrophication parameters and tropic state indices in 30 waste receiving Swedish Lakes, Archiv Hydrobiol, 89, 189- 207
  3. 이용우, 2001, HPLC를 이용한 곰소만 퇴적물 중의 저서미세조류의 색소 연구, 석사학위논문, 해양학 과, 부경대학교, 부산
  4. Bidigare R. R., Frank T. T., Zastrow C., Brooks J. M., 1986, The distribution of algal chlorophylls and their degradation products in the southern ocean, Deep Sea Research, 33, 923-937 https://doi.org/10.1016/0198-0149(86)90007-5
  5. Wright S. W., Jeffrey S. W., 2006, Pigment markers for phytoplankton production In: Marine Organic Matter: Biomarkers, isotopes and DNA, Springer- Verlag, Berlin, ISBN-10-3-540-28401-X, 71-104
  6. Park M. O., 2006, Composition and distribution of phytoplankton with size fraction results at South- western East/Japan Sea, Ocean Science Journal, 41(4), 301-313 https://doi.org/10.1007/BF03020632
  7. Montagna P. A., Blanchard G. F., Dinet A., 1995, Effect of production and biomass of intertidal microphytobenthos on meiofaunal grazing rates, Journal of Experimental Marine Biological Ecology, 185, 149-165 https://doi.org/10.1016/0022-0981(94)00138-4
  8. Anderson R. A., Bidigare R. R., Keller M. D., Latasa M., 1996, A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific Oceans, Deep Sea Research, 43, 517-537 https://doi.org/10.1016/0967-0645(95)00095-X
  9. Mackey M. D., Mackey D. J., Higgins H. W., Wright S. W., 1996, CHEMTAX-A program for estimating class abundances from chemical markers: Application to HPLC measurements of phytoplankton, Marine Ecology Progress Series, 144, 265-283
  10. 국립수산과학원, 2005, 해양환경공정시험방법, 55-65
  11. http://www.mathworks.com
  12. 이대인, 조현서, 2002, 가막만 해역의 오염부하 특 성에 관한 연구, 한국환경과학회지, 11(9), 945- 954
  13. 김귀영, 2002, 가막만의 이화학적 환경특성과 퇴적 물에서의 물질 거동 연구, 박사학위논문, 환경공학 과, 영남대학교, 대구
  14. 박성진, 2003, 가막만의 해양환경과 굴양식장에 미 치는 바람효과, 석사학위논문, 해양학과, 여수대학 교, 여수
  15. Guarini J. M., Blanchard G. F., Bacher C., Gros P., Riera P., Richard P., Gouleau D., Galois R., Prou J., Sauriau P. G., 1998, Dynamics of spatial patterns of microphytobenthic biomass: Inferences from a geostatistical analysis of two comprehensive surveys in Marennes-Oleron Bay(France), Marine Ecology Progress Series, 166, 131-141 https://doi.org/10.3354/meps166131
  16. Lewitus A. J., White D. L., Tymowski R. G., Geesey M. E., Hymel S. N., Noble P. A., 2005, Adapting the CHEMTAX method for assessing phytoplankton taxonomic composition in Southeastern U.S. estuaries, Estuaries, 28(1), 160-172 https://doi.org/10.1007/BF02732761
  17. 오승진, 문창호, 박미옥, 2004, 한국 서해 새만금 갯벌에서 저서미세조류의 생체량과 군집조성에 대 한 HPLC 분석, 한국수산학회지, 37(3), 215- 230
  18. Zapata M., Jeffrey S. W., Wright S. W., Rodriguez F., Clementson L., Garrido J. L., 2004, Photosynthetic pigments in 37 species(65 strains) of Haptophyta: Implications for phylogeny and oceanography, Marine Ecological Progress Series, 270, 83-102 https://doi.org/10.3354/meps270083