Effects of Macrophage on Biodegradation of β-tricalcium Phosphate Bone Graft Substitute

대식세포가 β-tricalcium Phosphate 뼈이식제의 생분해에 미치는 영향

  • Kim, Young-Hee (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Jyoti, Anirban (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Byun, In-Sun (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Oh, Ik-Hyun (Korea Institute of Industrial Technology(KlTECH), Gwangju Research Center) ;
  • Min, Young-Ki (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Yang, Hun-Mo (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Lee, Byong-Taek (Department of Biomedical Engineering & Materials, School of Medicine, Soonchunhyang University) ;
  • Song, Ho-Yeon (Department of Microbiology, School of Medicine, Soonchunhyang University)
  • 김영희 (순천향대학교 의과대학 미생물학교실) ;
  • ;
  • 변인선 (순천향대학교 의과대학 미생물학교실) ;
  • 오익현 (한국생산기술연구원 광주연구센터) ;
  • 민영기 (순천향대학교 의과대학 생리학교실) ;
  • 양훈모 (순천향대학교 의과대학 생리학교실) ;
  • 이병택 (순천향대학교 의과대학 의공학교실) ;
  • 송호연 (순천향대학교 의과대학 미생물학교실)
  • Published : 2008.10.31


Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $\beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $\beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $\beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $\beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $\beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $\beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $\beta$-TCP synthesized by fibrous monolithic process. The $\beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $\beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $\beta$-TCP bone graft substitutes.


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