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Effects of Macrophage on Biodegradation of β-tricalcium Phosphate Bone Graft Substitute

대식세포가 β-tricalcium Phosphate 뼈이식제의 생분해에 미치는 영향

  • Kim, Young-Hee (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Jyoti, Anirban (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Byun, In-Sun (Department of Microbiology, School of Medicine, Soonchunhyang University) ;
  • Oh, Ik-Hyun (Korea Institute of Industrial Technology(KlTECH), Gwangju Research Center) ;
  • Min, Young-Ki (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Yang, Hun-Mo (Department of Physiology, School of Medicine, Soonchunhyang University) ;
  • Lee, Byong-Taek (Department of Biomedical Engineering & Materials, School of Medicine, Soonchunhyang University) ;
  • Song, Ho-Yeon (Department of Microbiology, School of Medicine, Soonchunhyang University)
  • 김영희 (순천향대학교 의과대학 미생물학교실) ;
  • ;
  • 변인선 (순천향대학교 의과대학 미생물학교실) ;
  • 오익현 (한국생산기술연구원 광주연구센터) ;
  • 민영기 (순천향대학교 의과대학 생리학교실) ;
  • 양훈모 (순천향대학교 의과대학 생리학교실) ;
  • 이병택 (순천향대학교 의과대학 의공학교실) ;
  • 송호연 (순천향대학교 의과대학 미생물학교실)
  • Published : 2008.10.31

Abstract

Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $\beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $\beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $\beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $\beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $\beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $\beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $\beta$-TCP synthesized by fibrous monolithic process. The $\beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $\beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $\beta$-TCP bone graft substitutes.

References

  1. P. Ducheyne, Q. Qiu, "Bioactive Ceramics: the Effect of Surface Reactivity on Bone Formation and Bone Cell Function," Biomaterials, 20 2287-303 (1999) https://doi.org/10.1016/S0142-9612(99)00181-7
  2. J.E. Lee, J.C. Park, Y.H. Kim, and H. Suh, "In vitro Evaluation of PEG Modified Polyurethanes in Cellular Toxicity," Biomet. Res., 2 [2] 65-8 (1998)
  3. H. S. Kang, "Histology First Edition," pp. 115-145, Komoonsa, Korea, 1993
  4. S. A. Park, J. W. Shin, et al "In Vitro Study of Osteogenic Differentiation of Bone Marrow Stromal Cells on Heattreated Porcine Trabecular Bone Blocks.," Biomaterials, 25 [3] 527-35 (2004) https://doi.org/10.1016/S0142-9612(03)00553-2
  5. K. de Groot, "Calcium Phosphate Ceramics: their Current Status." pp. 477-92. In: J.W. Boretos and M. Eden, Editors, Contemporary biomaterials, Noyes Publications, USA, 1984
  6. M, Jarcho, "Calcium Phosphate Ceramics as Hard Tissue Prosthetics," Clin. Orthop., 157 259-78 (1981)
  7. J. W. Ha and H. J. Jung, "Preparation of Polycrystalline Hydroxyapatite Ceramics for the Application of Tooth Implants(in Korean)," J. Kor. Ceram. Soc., 20 [1] 55-62 (1983)
  8. Ogose A, Hotta T, Hatano H, Kawashima H, Tokunaga K, Endo N, and Umezu H., "Histological Examination of Beta0tricalcium Phosphate Graft in Human Femur.," J. Biomed. Mater. Res., 63 601-4 (2002) https://doi.org/10.1002/jbm.10380
  9. Ozawa M, Tanaka K, Morikawa S, Chazono M, and Fujii K., "Clinical Study of the Pure Beta-tricalcium Phosphate: Reports of 167 Cases., J. East. Jpn. Orthop. Traumatol., 12 409-13 (2000)
  10. Ozawa M., "Experimental Study on Bone Conductivity and Absorbability if the Pure $\beta$-TCP.," J. Jap. Soc. Biomat., 13 17-25 (1995)
  11. Chazono M, Tanaka T, Komaki H, and Fujii K., "Bone Formation and Bioresorption after Implantation of Injectable Btricalcium Phosphate Granules-hyaluronate Complex in Rabbit Bone Defects.," J. Biomed. Mater. Res., 70 [A] 542-9 (2004)
  12. Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N, et al. Bone "Formation and Resorption of Highly Purified $\beta$- tricalcium Phosphate in the Rat Femoral Condyle.," Biomaterials, 26 5600-8 (2005) https://doi.org/10.1016/j.biomaterials.2005.02.026
  13. Ogose A, Kondo N, Umezu H, Hotta T, Kawashima H, Tokunaga K, et al. "Histological Assessment in Grafts of Highly Purified Betatricalcium Phosphate (OSferions) in Human Bones.," Biomaterials, 27 1542-9 (2006) https://doi.org/10.1016/j.biomaterials.2005.08.034
  14. Ho-Yeon Song, Young-Ki Min, Hun-Mo Yang, and Joo- Yang Mang, "The Effects of HAp and BCP Nano Powders Synthesized by Microwave-assisted Synthesis on the Activation of Osteoblast and Osteoclast," Kor. J. Mater. Res., 17 [12] 669-75 (2007) https://doi.org/10.3740/MRSK.2007.17.12.669
  15. Hwang KK, Huh NK, and Lee JH., "Studies on the Signaling on the Molecules in RANK, an Osteoclast Differentiation Receptor," Oral Biol. Res., 24 245-54 (2000)
  16. Grigoriades AE, Wang ZQ, Cecchini MG, Hofstetter W, Felix R, Fleisch HA, and Wagner EF., "c-Fos: A Key Regulator of Osteoclast-macrophage Lineage Determination and Bone Remodeling," Science, 266 443-48 (1994)
  17. Teitelbaum SL., "Bone Resortion by Osteoclast.," Science, 289 1504-08 (2000) https://doi.org/10.1126/science.289.5484.1504
  18. Boyle WJ, Simonet WS, and Lacey DL., "Osteoclast Differentiation and Activation.," Nature, 423 337-42 (2003) https://doi.org/10.1038/nature01658
  19. I.-C. Kang, S.-H. Cho, H.-Y. Song, and B.-T. Lee, "Fabrication of Continuously Porous Alumina Bodies by Multiextrusion Process and their In-vitro and In-vivo Study for Biocompatibility(in Korean)," J. Kor. Ceram. Soc., 41 [7] 560-66 (2004) https://doi.org/10.4191/KCERS.2004.41.7.560
  20. K. A. Hing, S. M. Best, and W. Bonfield, "Characteristic of Porous Hydroxyapatite," J. Mater. in Med., 10 135-45 (1999) https://doi.org/10.1023/A:1008929305897
  21. L.L. Hench, "Bioceramics," J. Am. Ceram. Soc., 81 1705-28 (1998) https://doi.org/10.1111/j.1151-2916.1998.tb02540.x
  22. S. Higashi, T. Yamamuro, T. Nakamura, Y. Ikada, S.H. Hyon, and K. Jamshidi, "Polymer-hydroxyapatite Composites for Biodegradable Bone Fillers," Biomaterials, 7 [3] 183-87 (1986) https://doi.org/10.1016/0142-9612(86)90099-2
  23. F.H. Albee, "Studies in Bone Growth-triple Calcium Phosphate as a Stimulus to Osteogenesis," Ann. Sur., 71 33-7 (1920)

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