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Study of Distance Relationships among Domestic Radish (Raphanus sativus L.) by Analyzing its Anti-fungal Protein Gene.

항 곰팡이 단백질 유전자 분석에 의한 국내 무 품종간 유연성에 관한 연구

  • Hwang, Cher-Won (School of Global Leadership (Institutes of Bioscience and Technology), Handong Global University)
  • 황철원 (한동대학교 기초학부(생명과학연구소))
  • Published : 2007.09.30

Abstract

To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it's raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish's anti- fungal protein[15].

References

  1. Broekaert, W. F., B. P. A. Cammue, M. F. C. De Bolle, K. Thevissen, C. W. De Samblanx and R. W. Osborn. 1997. Antimicrobial peptides from plants. Crit. Rev. plant Sci. 16, 297-323 https://doi.org/10.1080/07352689709701952
  2. Choi, Y. H. and C. H. Hwang. 2005. The biochemical properties of Korean radish (Raphanus satious L.) and its seed protein as a natural preservative: The influences on antigenotoxicity and cytootoxicity. J. Life Science 15(4), 561-565 https://doi.org/10.5352/JLS.2005.15.4.561
  3. Colilla, F. J., A. Rocher and E. Mendez. 1990. Gamma purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm. FEBS Lett. 270, 191-194 https://doi.org/10.1016/0014-5793(90)81265-P
  4. Douliez, J. P., S. Jegou, C. Pato, C. Larre, D. Molle and D. Marion. 2001. Identification of a new form of lipid transfer protein (LTP1) in wheat seeds. J. Agric. Food Chem. 49, 1805-1808 https://doi.org/10.1021/jf001327m
  5. Garcia, O. F., P. Rodriguez Palenzuela, A. Molina, J. M. Alamillo, E. Lopez Solanilla, M. Berrocal Lobo and C. Poza Carrion. 2001. Antibiotic activities of peptides, hydrogen peroxide and peroxynitrite in plant defense. FEBS Lett. 498, 219-222 https://doi.org/10.1016/S0014-5793(01)02456-5
  6. Hnacock, R. E. W. and R. Lehrer. 1998. Cationic peptides: a new source of antibiotics. TBTECH 16, 82-86 https://doi.org/10.1016/S0167-7799(97)01156-6
  7. Hur, M. K. and Oh. Ohnish. 2003. Genetic diversity and relationship among natural and cultivated population of radish in Korea revealed by RAPD. Korean J. Genetics 25(2), 119-125
  8. Kim, K. Y., M. Davison and H. J. Chung. 2000. Antimicrobial effectiveness of pine needle extract on food borne illness bacteria. J. Microbiol. Biotechnol. 10, 227-232
  9. Leah, R., H. Tomrnerup, I. Svendsen and J. Mundy. 1991. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem. 266, 1564-1573
  10. Osborn, R. W, G. W. De Samblanx, K. Thevisson, I. Goderis, S. Torrekens, F. Van Leuven, S. Attenborough, S. B. Rees and W. F. Broekaert. 1995. Isolation and characterization of plant defensin from seeds of Asieraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 368, 257-262 https://doi.org/10.1016/0014-5793(95)00666-W
  11. Park, J. H., H. K. Shin and C. W. Hwang. 2001. New antimicrobial activity from Korean radish seeds (Raphanus sativus L.) J. Microbiol. Biotechnol. 11, 337-341
  12. Selitrennikoff, PC. 2001. Antifungal proteins. Appl. Environ. Microbiol. 67, 2883-2894 https://doi.org/10.1128/AEM.67.7.2883-2894.2001
  13. Seo, Y. W., K. W. Cho, H. S. Lee, T. M. Yoon and J. H. Shin. 2000. New polyene macrolide antibiotics from Streptomyces sp. M90025. J. Microbiol. Biotechnol. 10, 176-180
  14. Terras, F. R. G., H. M. E. Schoofs, M. F. C. De Bolle, F. Van Leuven, S. B. Rees, J. Vanderleyden, B. P. A. Cammue and W. F. Broekaert. 1992. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus satious L.) seeds. J. Biol. Chem. 267, 15301-15309
  15. Tsezi, A. Egorov, O. Tatyana I., P. Vitaliy A. and G. Eugene V. 2005. Diversity of wheat anti - microbial peptide. PEPTIDE 26, 2064-2073 https://doi.org/10.1016/j.peptides.2005.03.007
  16. Ovalia, M., C. C. Hager and T. N. Oeltmann. 1990. An antimelanoma - barley ribosome inactivating protein conjugate is cytotoxic to melanoma cells in vitro. Anticancer Drug 10, 671-675 https://doi.org/10.1097/00001813-199908000-00007