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Characteristics of Bacterial Community for Biological Activated Carbon(BAC) by Culturable and Unculturable Methods.

배양적 및 비배양적 방법에 의한 생물활성탄 부착세균 군집 특성

  • Park, Hong-Ki (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Eun-Young (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Mi-Eun (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Jong-Moon (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Ji, Ki-Won (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Yu, Pyung-Jong (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
  • 박홍기 (부산광역시 상수도사업본부 수질연구소) ;
  • 정은영 (부산광역시 상수도사업본부 수질연구소) ;
  • 정미은 (부산광역시 상수도사업본부 수질연구소) ;
  • 정종문 (부산광역시 상수도사업본부 수질연구소) ;
  • 지기원 (부산광역시 상수도사업본부 수질연구소) ;
  • 유평종 (부산광역시 상수도사업본부 수질연구소)
  • Published : 2007.09.30

Abstract

The Biological Activated Carbon (BAC) process in the water treatments represents a kind of biofiltration process which capabilities of bacteria to remove organic matters are maximized. It enables to eliminate organic matters and effectively reduce microbial regrowth potentials. As attached bacteria employ natural organic matter as a substrate, they are significantly dependent on indigenous microorganisms. In this study, characteristics of bacterial community by culturable and unculturable Methods have been conducted in a pilot plant using SAC in water treatment process at the downstream of the Nakdong River. Based on the results, HPC and bacterial- production for coal-based activated carbon material were $1.20{\sim}56.2{\times}l0^7$ cfu/g and $1.2{\sim}3.7\;mgC/m^{3}h$, respectively, in the SAC process. The highest level of attached bacteria biomass and organic carbon removal efficiency was found in the coal-based activated carbon. The genera Pseudomonas, Flavobacterium, Alcaligenes, Acilzetobacter, and Spingomonas were identified for each activated carbon material. Pseudomonas vesicularis was the dominant species in the coconut- and coal-based materials, where as Pseudomonas cepacia was the dominant species in the wood-based material. The Scanning Electron Microscope (SEM) observation of the activated carbon surface also found the widespread distribution of rod form and coccus. The community of attached bacteria was investigated by performing Fluorescent in situ hybridization (FISH) analysis. a group was dominant in coal, wood and coccunt-based materials, ${\alpha},\;{\beta}\;and\;{\gamma}$ group ranged from 27.0 ${\sim}$ 43.0%, 7.1 ${\sim}$ 22.0%, 11.3 ${\sim}$ 28.6%, respectively. These results suggest that a group bacterial community appears to be regulated removal efficiency of organic material in water treatment process.

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