The Change in Hematological Factors of Ascidian (Styela clava) Extract Diets Fed Rainbow Trout (Oncorhynchus mykiss).

미더덕 추출액을 섭취한 무지개 송어 혈액성상의 변화

  • Jeong, U-Cheol (Division of Marine Life Science, Gyeongsang National University) ;
  • Lee, Jeong-Tae (Division of Marine Life Science, Gyeongsang National University) ;
  • Palmos, Grace N (Division of Marine Life Science, Gyeongsang National University) ;
  • Kang, Seok-Joong (Institute Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dae (Institute Marine Industry, Gyeongsang National University)
  • 정우철 (경상대학교 해양생명과학부) ;
  • 이정태 (경상대학교 해양생명과학부) ;
  • ;
  • 강석중 (경상대학교 해양산업연구소) ;
  • 최병대 (경상대학교 해양산업연구소)
  • Published : 2007.09.30


Effects of muscle fluid concentrates from Styela clava on fish diets were investigated in rainbow trout(Oncorhynchus mykiss). Fish were fed on one of the isonitrogenous(48%) and isolipic(20%) feed containing 5 to 20% of muscle fluid concentrates for 8 weeks. Hematological parameters such as hemoglobin, hematocrit, albumin, glucose, total bililubin, triglyceride and glutamic pyruvate transaminase of the rainbow trout which were fed on the diets varied, but no specific trend became apparent. However, glutamic oxaloacetic transaminase value was significantly higher than that of normal fishes. Therefore, concentrated ascidian fluid which is normally discarded, can be combined with commercial diets for the normal growth of rainbow trout.


  1. Cho, K. S., E. Y. Min and J. C. Kang. 2002. Changes of hematological constituents in the nile tilapia, Oreochromis niloticus exposed to HCB and PCBs. J. Korean Fish Soc. 35, 110-114
  2. Choi, H. S., S. R. Park and C. G. Jung. 2002. Biochemicalanalysis of blood serum from wintering seabream with green liver syndrome. J. Fish Pathol. 15, 43-48
  3. Gabriel, U. U., G. N. O. Ezeri and O. O. Opabunmi. 2004. Influence of sex, source, health status and acclimation on the hematology of Clarias gariepinus (Burch, 1822). Afr. J. Biotechnol. 3, 463-467
  4. Gandra, M., M. C. M. Cavalcante and M. S. G. Pavao. 2000. Anticoagulant sulfated glycosaminoglycans in the tissues of the primitive chordate Styela plicata (Tunicate). Glycobiology 10, 1333-1340
  5. Jang, S. I., Y. J. Jo and J. S. Lee. 1992. Effects of vitamins and glycyrrhizin added to oxidized diets on the growth and on the resistance to Edward siella infection of nile tila-pia, Oreochromis niloticus. J. Aquacult. 5, 143-155
  6. Lee, K. H, Y. S. Lee, J. H. Kim and D. S. Kim. 1998. Utilization of obosan (dietary herbs) II, Muscle quality of olive flounder, Paralichthys olivaceus fed with diet containing obosan. J. Aquacult. 11, 319-325
  7. Lee, S. M., D. J. Kim, J. K. Kim, S. B. Hur, J. K. Lee and H. K. Lim. 2000. Effects of Kluyveromyces fragilis, Candida utilis and brewer's yeast as an additive in the diet on the growth and body composition of juvenile Korean rockfish (Sebastes schlegeli). J. Korean Fish Soc. 33, 463-468
  8. Lindsay, G. J. H., M. J. Walton, J. W. Adron, T. C. Fletcher, C. Y. Cho and C. B. Cowey. 1984. The growth of rainbow trout (Salmo gairdneri) given diets containing chitin and its relationship to chitinolytic enzymes and chitin digestibility. Aquaculture 37, 315-334
  9. Matuso, K. and I. Miyazono. 1993. The influence of long-term administration of peptidoglycan on disease resistance and growth of juvenile rainbow trout. Nippon Suisan Gakkaishi 59, 1377-1379
  10. Nakagawa, H. S., E. Kasahara, T. Duo, T. Minami and K. Akira. 1981. Effect of Chlorella extract supplement in diet on resisting power against disease of culture ayu. Aquaculture 29, 109-116
  11. Nematipour, G. R., H. Nakagawa, K. Nanba, S. Kasahara, A. Tsujimura and K. Akira. 1987. Effect of Chlorella-extract supplement to diet on lipid accumulation of ayu. Nippon Suisan Gakkaishi 53, 1687-1692
  12. Park, S. U. M. G. Kwon, Y. H. Lee, K. D. Kim, I. S. Shin and S. M. Lee. 2003. Effects of supplemental Undaria, obosan and wasabi in the experimental diets on growth, body composition, blood chemistry and non-specific immune response of juvenile flounder Paralichthys olivaceus. J. Aquacult. 16, 210-215
  13. Satho, K. I., H. Nakagawa and S. Kasahara. 1987. Effect of Ulva meal supplementation on disease resistance of red sea bream. Nippon Suisan Gakkaishi 53, 1115-1120
  14. Shiau, S. Y. and Y. P. Yu. 1999. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloiicus x O. aureus. Aquaculture 179, 439-446
  15. Wang, Y., C. F. Chang, J. Chou, H. L. Chen, X. Deng, B. K. Harvey, J. L. Cadet and P. C. Bickford. 2005. Dietary supplementation with blueberries, spinach, or Sptrulina reduces ischemic brain damage. Exp. Neurol. 193, 75-84
  16. Zhou, Z. P., L. N. Liu, X. L. Chen, J. X. Wang, M. Chen, Y. Z. Zhang and B. C. Zhou. 2005. Factors that effect antioxidant activity of C-phycocyanins from Spirulina platensis. J. Food Bichem. 29, 313-322
  17. Kim, D. S., J. H. Kim, C. H. Jong, S. Y. Lee, S. M. Lee and Y. B. Moon. 1998. Utilization of obosan (dietary herbs) I. Effects on survival, growth, feed conversion ratio and condition factor in olive flounder, Paralichthys olivaceus. J. Fish Pathol. 11, 213-221