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Antioxidaive Activity of Lotus Root(Nelumbo nucifera G.) Extracts.

연근 추출물의 항산화 효과

  • 이재준 (조선대학교 식품영양학과) ;
  • 하진옥 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2007.09.30

Abstract

This study was investigated on the antioxidant capacity of 80% ethanol extract of lotus root in vitro. The ex-traction yields of 80 % ethanol extract was 9.14%. Lotus root ethanol extract was fractionated by the following: n-hexane, chloroform, ethylacetate and n-butanol. n-Butanol fraction showed the highest ex-traction yield of all fractions. Antioxidative activities of different fractions were examined by l.l-diphenyl-2-picrylhydrazyl(DPPH) radical generation, the Rancimat test, the nitrite scavenging ac-tivity and the thiobarbituric acid(TBA) method, and compared with the properties of the commercial antioxidant butylated hydroxytoluene(BHT). The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol. Furthermore, the antioxidative capacity of the ethylacetate fraction was similar to that of BHT. In conclusion, these results suggest that lotus root may be a good candidate as a natural antioxidant source.

References

  1. Antolovich, M., P. D. Prenzler, E. Patsalides, S. McDonald and K. Robards. 2003. Methods for testing antioxidant activity. Analyst 127, 183-198 https://doi.org/10.1039/b009171p
  2. A.O.A.C. 1990. Official Methods of Analysis. pp. 8-15. 15th ed. Association of official analytical chemists. Washington DC
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200
  4. Borsch, T. and W. Barthlott. 1983. Classification and distribution of the genus Nelumbo adans (Nelumbonaccae). Beitr. Biol. Plazen 68, 421-450
  5. Borrello, S., A. Seccia, T. Galleotti, G. M. Bartoli, E. Farallo and F. Serri, 1984. Protective enzymes in human epidermal carcinomas and psoriasis. Arch. Dermatol. Res. 276, 338-340 https://doi.org/10.1007/BF00404630
  6. Cho, E. J., T. Yokozawa, D. Y. Rhyu, S. C. Kim, N. Shibahara and J. C. Park. 2003. Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical. Phytomedicine 10, 544-551 https://doi.org/10.1078/094471103322331520
  7. Cho, S. H, R. K. Kang and H. G. Lee. 1984. A study on the ingredients preparation method of lotus root jung kwa. J. Korean Soc. Food Sci. Nutr. 13, 42-50
  8. Cho, S. I. and H. W. Kim. 2003. Beneficial effect of Nodus neoumbinis nhizomatis extracts on cisplatin-induced kidney toxicity in rats. Korean J. Herbology 18, 127-134
  9. Chung, K. S., H. S. Yun-Choi and Y. Hahn. 2000. High performance liquid chromatographic analyses of higenamine enatiomers in aconite roots. Natural Product Sci. 6, 20-24
  10. Esquivel, M. M., M. A. Ribeiro and M. G. BermardoGil. 1999. Supercritical extraction of savory oil : study of antioxidant activity and extract characterization. J. Super critical Fluids 14, 129-138 https://doi.org/10.1016/S0896-8446(98)00115-6
  11. Gray, J. I. and J. R. Dugan. 1975. Inhibition of n-nitrosamine formation in model food systems. J. Food Sci. 40, 981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  12. Harman, D. 1987. The free radical theory of aging. In modern biological theories of aging. pp. 89. Warner, H. R., R. N. Butler, R. L. Sprott, E. L. Schneider, eds. Raven Press NY
  13. Hong, J. Y., H. S. Nam, Y. S. Lee, K. Y. Yoon, N. W. Kim and S. R. Shin. 2006. Study on the antioxidant activity of extracts from the fruit of Elaeagnus multiflora thunb. Korean J. Food Reserv. 13, 413-419
  14. Hu, M. H and L. H. Skibsted. 2002. Antioxidative capacity of rhizome extract and rhizome knot extract of edible lotus(Nelumbo Nucifera). Food Chem. 76, 327-333 https://doi.org/10.1016/S0308-8146(01)00280-1
  15. Hur, S. K., S. S. Kim, Y. H. Heo, S. M. Ahn, B. G. Lee and S. K. Lee. 2001. Effect of the grapevine shoot extract on free radical scavenger activity and inhibition of pro-inflammatory mediator production in raw macrophages. J. Applied Pharmacol. 9, 188-193
  16. Jae, J. U. 1989. Isolation and identification of constituents from antioxidant and hematopoietic fractions of panax ginseng CA. Mayer. Ph D Thesis. Seoul University
  17. Joo, K. L. and J. J. Kim. 2002. Oxidative stability and compounds of sesame oils blended with vegetable oils. Korean J. Food Sci. Technol. 34, 499-502
  18. Jung, G. T., I. O. Ju, J. S. Choi and J. S. Hong. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT(Omija) seed. Korean J. Food Sci. Technol. 32, 928-935
  19. Jung, H. A., J. E. Kim, H. Y. Chung and J. S. Choi. 2003. Antioxidant principles of Nelumbo nucifera stamens. Arch. Pharm. Res. 26, 279-285 https://doi.org/10.1007/BF02976956
  20. Kang, Y. H, Y. K. Park and G. D. Lee. 1996. The nitrite scavenging and electron donating ability of polyphenolic compounds(in Korean). Korean J. Food Sci. Technol. 28, 232-239
  21. Kato, H., C. Lee, N. V. Kim and S. B. Hayase. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins, Agric. Bio. Chem. 51, 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  22. Kim, J. S. 2002. Anti-diabetic constituent from the node of lotus rihzome(Nelumbo nucifera Gaertn). MS Thesis. ChungAng University
  23. Kim, Y. S., S. S. Jeon and S. T. Jung. 2002. Effect of lotus powder on the baking quality of white bread. Korean J. Soc. Food Cookery Sci. 18, 413-425
  24. Ko, B. S., D. W. Jun, J. S. Jang, J. H. Kim and S. Park. 2006. Effect of Sasa Borealis and white lotus roots and leaves on insulin action and secretion in vivo. Korean J. Food Sci. Technol. 38, 114-120
  25. Lee, J. J., S. E. Park and M. Y. Lee. 2006. Effects of lotus root(Nelumbo nucifera G.) on lipid metabolism in rats with diet-induced hypercholesterolemia. Korean J. Food Preserv. 13, 634-642
  26. Lee, J. J., S. E. Park, Y. M. Lee and M. Y. Lee. 2006. Protective effects of lotus root(Nelumbo nucifera G.) on hepatic injury induced by alcohol in rats. Korean J. Food Preserv. 13, 774-782
  27. Lee, K. D., H. K. Chang and H. K. Kim. 1997. Antioxidative and nitrite scavenging activities of edible mushrooms. Korean J. Food Sci. Technol. 29, 432-436
  28. Lee, K. S., M. G Kim and K. Y. Lee. 2004. Antimicrobial effect of the extracts of cactus Chounnyouncho(Opuntia humifusa) aganist food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33, 1268-1272 https://doi.org/10.3746/jkfn.2004.33.8.1268
  29. Lee, M. W., J. S. Kim, S. M. Cho, J. H. Kim and J. S. Lee. 2001. Anti-diabetic constituent from the node of lotus rhizome(Nelumbo nucifera Gaertn). Natural Product Sci. 7, 107-109
  30. Ling, Z. Q., B. J. Xie and E. L. Yang. 2005. Isolation, characterization, and determination of antioxidative of oligomeric procyanidins from the seedpod of Nelumbo nucifera Gaertn. J. Agric. Food Chem. 53, 2441-2445 https://doi.org/10.1021/jf040325p
  31. Moon, S. M., H. J. Kim and K. S. Ham. 2003. Purification and characterization of polyphenol oxidase from lotus root(Nelumbo nucifera G). Korean J. Food Sci. Technol. 35, 791-796
  32. Na, M. K., R. N. An, S. M. Lee, N. D. Hong, J. K. Yoo, C. B. Lee, J. P. Kim and K. H. Bae. Screening of crude drugs for antioxidative activity. Kor. J. Phannacogn. 32, 108-115
  33. Ottolenghi, A. 1959. Interaction of ascorbic acid and mitochondrial lipids. Arch. Biochem. Biophy. 79, 355-461 https://doi.org/10.1016/0003-9861(59)90414-X
  34. Paker, L. and A. N. Glazer. 1990. Oxygen radicals in biological systems. In Methods in Enzymology. Oxygen radicals in biological systems. pp. 186-343. Academic Press, London
  35. Park, S. H, E. H. Shin, J. G. Koo, T. H. Lee and J. H. Han. 2005. Effects of Nelumbo nucifera on the regional cerebral blood flow and blood pressure in rats. J. East Asian Soc. Dietary Life 15, 49-56
  36. Park, S. H., T. S. Hahm and J. H Han. 2005. Effects of ethanol-extract of lotus root on the renal function and blood pressure of fructose-induced hypertensive rats. J. East Asian Soc. Dietary Life 15, 165-170
  37. Park, S. H, J. S. Hyun, E. H. Shin and J. H. Han. 2005. Functional evaluation of lotus root on serum lipid profile and health improvement. J. East Asian Soc. Dietary Life 15, 257-263
  38. Quan, J. Q. 2002. Cardiovascular pharmacological effects of bibenzylisoquinoline alkaloid derivatives. Acta. Pharmacol. Sin. 23, 1086-1092
  39. Sang, D., J. H. Kim and J. O. Hoon. 1981. Antioxidant activity of panax ginseng browning products. J. Korean Agri. Chem. Soc. 24, 161-166
  40. Won, H. T. 2004. Effects of Nodus neoumbinis nhizomatis extracts on sociopsychological stress in mice. MS Thesis. Dongshin University
  41. Han, S. J. and S. J. Koo. 1993. Study of the chemical composition in bamboo, shoot, lotus root and burdock. Korean J. Soc. Food Sci. 9, 82-87
  42. Xiao, J. H., J. H. Zhang., H. L. Chen., X. L. Feng and J. L. Wang. 2005. Inhibitory effects of isoliensinine on bleomycin-induced pulmonary fibrosis in mice. Planta Med. 71, 225-227 https://doi.org/10.1055/s-2005-837821
  43. Park, I. B., J. W. Park, J. M. Kim, S. T. Jung and S. G. Kang. 2005. Quality of soybean paste(Doenjang) prepared with lotus root powder. J. Korean Soc. Food Sci. Nutr. 34, 519-523 https://doi.org/10.3746/jkfn.2005.34.4.519
  44. Kang, Y. H, Y. K. Park, S. R. Oh and K. D. Moon. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984
  45. Cha, G. S. and C. U. Choi, 1990. Determination of oxidation stability of perilla oil by the Rancimat method. Korean J. Food Sci. Technol. 22, 61-65
  46. Yang, H. C, Y. H. Kim, T. K. Lee and Y. S. Cha. 1985. Physicochemical properties of lotus root(Nelumbo nucifera G.) starch. J. Korean Agri. Chem. Soc. 28, 239-244
  47. Kytopoulos, S. A. 1987. Ascorbic acid and formation of n-nitroso compounds: possible role of ascorbic acid in cancer prevention. Am. J. Clin. Nutr. 45, 1344-1350 https://doi.org/10.1093/ajcn/45.5.1344
  48. Lee, K. S., M. G. Kim and K. Y. Lee. 2006. Antioxidative activity of ethanol extract from lotus(Nelumbo nucifera) leaf. J. Korean Soc. Food Sci. Nutr. 35, 182-185 https://doi.org/10.3746/jkfn.2006.35.2.182
  49. Kim, S. B., S. B. Rho, D. Y. Rhyu and D. W. Kim. 2005. Effect of Nelumbo nucifera leaves on hylerlipidemic and atherosclerotic bio F1B hamster. Korean J. Pharmacogn. 36, 229-234

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