Cooperative Induction of HL-60 Cell Differentiation by Combined Treatment with Eugenol and 1α,25-Dihydroxyvitamin D3

Eugenol과 1α,25-dihydroxyvitamin D3의 병합처리에 의한 HL-60 세포의 분화 유도

  • Oh, Mi-Kyung (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Park, Seon-Joo (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Kim, Nam-Hoon (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Cho, Jin-Kyung (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Jin, Jong-Youl (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Kim, In-Sook (Department of Natural Sciences, College of Medicine, The Catholic University of Korea)
  • 오미경 (가톨릭대학교 의과대학 자연과학교실) ;
  • 박선주 (가톨릭대학교 의과대학 자연과학교실) ;
  • 김남훈 (가톨릭대학교 의과대학 자연과학교실) ;
  • 조진경 (가톨릭대학교 의과대학 자연과학교실) ;
  • 진종률 (가톨릭대학교 의과대학 내과) ;
  • 김인숙 (가톨릭대학교 의과대학 자연과학교실)
  • Published : 2007.09.30


Eugenol (4-allyl-2-methoxyphenol) is a main component of essential oils obtained from various spices. Recent reports have shown that eugenol induces growth inhibition and apoptosis of malignant tumor cells. In this study, the stimulatory effect of eugenol on cell differentiation was investigated in HL-60 promyelocytic leukemia cells. When HL-60 cells were treated in combination with 150 ${\mu}M$ of eugenol and 3 nM of $1{\alpha},25-dihydroxyvitamin$ $D_{3}$, cell growth was slower than that of cells treated with eugenol or $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ alone. Eugenol enhanced low dose of $1{\alpha,25-dihydroxyvitamin }$ $D_{3}-induced$ a $G_{0}/G_{1}$ phase arrest in cell cycle. Consistent with this, combined treatment of eugenol and $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ cooperatively increased p27 level and decreased cyclin A, cdk 2 and cdk 4 levels, which are cell cycle regulators related to $G_{0}/G_{1}$ arrest. According to flow cytometric analysis, the expression of CD14 (monocytic differentiation marker) was more increased in the cells co-treated with eugenol and $1{\alpha},25-dihydroxyvitamin$ $D_{3}$. These results indicate that eugenol potentiates cell differentiation mediated by $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ of suboptimal concentration. The differentiation-inducing property of eugenol maybe contributes to chemopreventive activity of cancer.


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