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Anti-tumor effect of Euphorbia hirta on Ehrlich's ascites carcinoma in mice

  • Kumarappan, CT (Division of Pharmacognosy, Department of Pharmaceutical Technology, Faculty of Engineering and Technology, Jadavpur University) ;
  • Sunderi, S Karpagam Kumara (Periyar College of Pharmaceutical Sciences, Department of Pharmacology) ;
  • Jaswanth, A (Periyar College of Pharmaceutical Sciences, Department of Pharmacology) ;
  • Lakshmi, S Mohana (Division of Pharmacognosy, Department of Pharmaceutical Technology, Faculty of Engineering and Technology, Jadavpur University) ;
  • Mandal, Subhash C (Division of Pharmacognosy, Department of Pharmaceutical Technology, Faculty of Engineering and Technology, Jadavpur University)
  • Published : 2007.06.30

Abstract

Anti-tumor activity of Euphorbia hirta (50 mg/kg and 100 mg/kg) has been evaluated against Ehrlich's ascites carcinoma (EAC) in Swiss albino mice. Intraperitoneal (i.p) administration of Euphorbia hirta was effective in reducing solid tumor mass development induced by EAC cells. It exhibited significant anti-tumor activity in mice, when used at the dose of 100 mg/kg/day i.p., for 14days. The administration of Euphorbia hirta (100 mg/kg/day i.p.) resulted in an increase (P<0.001) of the life span (59.9%) of ascites tumor bearing mice as compared to the control group. After 14 days, on developed tumor masses, Euphorbia hirta administration brought about significant reduction in tumor volume and it reverse the changes in the hematological parameters, responding to tumor inoculation. The results are indicative of the anti-tumor activity of Euphorbia hirta against EAC induced tumor in a dose dependent manner.

References

  1. Anonymous. (1959) In: The Wealth of India (Raw Materials), Vol. V, Council of Scientific and Industrial Research Publication (CSIR), New Delhi, pp. 295
  2. Ajao AO, Emele F, Femi Onadeko B. (1985) Antibacterial activity of Euphorbia hirta. Fitoterapia 56, 165-167
  3. Ambasta SP. (1986) In: The Useful Plants of India, Publication and Information Directorate, Council of Scientific and Industrial Research, New Delhi, pp. 302
  4. Armitage P, Berry G, Matthews JNS. (2001) In: Statistical Methods in Medical Research, 4th edition, Blackwell scientific publication, Oxford, pp. 100
  5. Bas Las RK, Agarwal R. (1980) Isolation and characterization of Euphorbia hirta Linn. Curr. Sci. 49, 311-312
  6. Brown JP. (1980) A review of the genetic effect of naturally occurring flavonoids, anthroquinones and related compounds. Mutat. Res. 75, 243-247 https://doi.org/10.1016/0165-1110(80)90029-9
  7. Caltagirone S, Rossi C, Poggi A, Ranelletti FO, Natali PG, Brunetti M, Aiello FB. (2000) Flavonoids apigenin and quercetin inhibit melanoma growth and metastatic potential. Int. J. Cancer 87, 595-600 https://doi.org/10.1002/1097-0215(20000815)87:4<595::AID-IJC21>3.0.CO;2-5
  8. Chaudhuri T, Sur P, Gomes A, Das SK, Ganguly DK. (1998) Effect of tea root extract (lRE) on solid tumors induced by 3-methylcholanthrene in mice. Phytother. Res. 12, 62-64 https://doi.org/10.1002/(SICI)1099-1573(19980201)12:1<62::AID-PTR184>3.0.CO;2-4
  9. Clarkson BD, Burchenal JH. (1965) Preliminary screening of antineoplastic drugs. Prog. Clin. Cancer 1, 625-629
  10. Cotran RS, Kumar V, Robins SL. (1994) In: Pathological Basis of Disease, 5th edition, W.B. Saunders Publication, U.S.A., pp. 241-256
  11. Dacie JV, Lewis SM. (1958) In: Practical Hematology, 2nd edition, London J & A Churchill Ltd., London, pp. 38-48
  12. D Amour FF, Blood, FR Belden DA. (1965) In: The manual for laboratory work in manunalian physiology, 3rd edition, The University of Chicago Press, Chicago, pp. 4-6
  13. Das T. (2004) Black tea induced cancer regression and amelioration of immunosuppression of the tumor bearer. A mechanistic approach, Proceedings of 23rd Annual Convention of the Indian Association for Cancer Research, 29th-31st January, pp. 39, Mumbai, India
  14. De Groot H. (1994) Reactive oxygen species in tissue injury. Hepatogastroenterology 41, 328-332
  15. Emerit I. (1994) Reactive oxygen species, Chromosome mutation, and Cancer: possible role of clastogenic factors in carcinogenesis. Free Radlc. BioI. Med. 16, 99-109
  16. Filleur F, Lebali C, Puroux JL, Simon A, Chulia AJ. (2001) Antiproliferative, anti-aromatous, anti-17b-HSD and anti-oxidant activity of lignans isolated from Myristica argentieamall (Myristacene). Planta Med. 67, 700-704
  17. Fotis T, Pepper MS, Aktan E, Breit S. (1997) Flavonoids, dietary derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res. 57, 2916-2918
  18. Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocete MA, Jimenez J. (1993) Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavanoid constituent. Planta Med. 15, 113-125
  19. Gothoskar SV, Ranadive KJ. (1971) Anti-cancer screening of SAN-AB. An extract of marking nut Semicarpus anacardium, Indian J. Exp. BioI. 9, 372 -375
  20. Grace P A. (1994) Ischaemia- perfusion injury. Br. J. Surg. 81, 637-647 https://doi.org/10.1002/bjs.1800810504
  21. Hirano T, Oka K, Akiba M. (1989) Anti-proliferative effect of synthetic and naturally occurring flavonoids on tumor cells of human breast carcinoma cell lines ZR-75-1. Res. Commun. Chem. Pathol. Pharmacol. 64, 69-78
  22. Hollman PC, Van Trijp JM, Buysman MN, Vander Gaag MS, Mengelers MJ, de Vreies JH, Katan MB. (1997) Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Lett. 41, 152-156 https://doi.org/10.1016/S0014-5793(97)01367-7
  23. Hudson L, Hay FC. (1989) isolation of normal peritoneal macrophages. In: Practical Immunology. 3rd Edition, Blackwell Scientific Publications., Oxford, London, pp.26-28
  24. Husain SR, Collard J, Collard P. (1987) Hydroxy radical scavenging activity of flavonoids. Phytochemistry 26, 2489-2492 https://doi.org/10.1016/S0031-9422(00)83860-1
  25. Jhonson PB, Abdurahman EM, Tiam EA, Abdu-Aguye I, Hussain IM. (1999) Euphorbia hirta leaf extracts increases urine output and electrolytes in rats. J. Ethnopharmacol. 65, 63-69 https://doi.org/10.1016/S0378-8741(98)00143-3
  26. Khandiya KL, Sangair RK, Bharadwaj A. (2002) Caffeine, quercetin and arizorin stimulate the exhalation of metabolic products of 14C-N-nitro diethyl amine induced lung tumerogenesis in mice. Indian J. Exp. BioI. 40, 739-740
  27. Kim BP, Mani I, Iversen L, Ziboh VA. (1998) Effects of naturally occurring flavonoids and bioflavoniods on epidermal cyclooxygenase and lipoxygenase from guinea-pigs. Prostaglandins Leukot. Essent. Fatty Acids 58, 17-24 https://doi.org/10.1016/S0952-3278(98)90125-9
  28. Kirtikar, KR, Basu BD. (1975) Indian Medicinal Plants, 2nd edition, Vol. III, M/S Periodical Experts, New Delhi, pp. 2197-2199
  29. Korkina LG, Afanas'ev lB. (1997) Antioxidant and chelating properties of flavonoids. Adv Pharmacol. 38, 151-163
  30. Lowry OH, Rosenbrough NT, Farr AL. (1951) Protein measurement with folin-phenol reagent. J. Biol. Chem. 173, 265-275
  31. Martinez-vazoquz M, Apan Tor, Lazcano ME, Bye R. (1999) Anti-inflammatory active compounds from the n-hexane extract of Euphorbia hirta. Rev. Soc. Quim. Mex. 13, 103-105
  32. Mazumdur UK, Malaya Gupta Maiti S, Mukherjee D. (1997) Antitumour activity of Hydrophilla spinosa on Ehrlich Ascites Carcinoma and Sarcoma- 180 induced mice. Indian J. Exp. BioI. 35, 473-477
  33. Nadir O, Pousset JL (1981) African Medicinal Plants VII - Invitro testing of Euphorbia hirta against Entamoeba Histolytica. Planta Med. 15, 113-125
  34. Oberling C, Guerin M. (1994) The role of virus as in the production of cancer. Adv. Cancer Res. 2, 353-423
  35. Polasa K. (2000) Chemoprevention of cancer: Dietary approach. Amala Res. Bull. 20, 49-53
  36. Prasad SB, Girl A. (1994) Antitumour effect of cisplatin against murine Ascites Dalton's Lymphoma. Indian J. Exp. BioI. 32, 155-162
  37. Rajkapoor B, Jayakar B, Murugesh, N. (2003) Antitumour Activity of Bauhinia variegata AgainstEhrlich Ascites Carcinoma Induced Mice. Pharm. BioI. 41, 604-607
  38. Ramnath V, Kuttan G, Kuttan R. (2002) Antitumour effect of Abrin (Abrus precatorious) on transplanted tumors in mice. Indian J. Exp. BioI. 46, 69-77
  39. Ram Sevak RS, Dewiti DL, Nair MG. (2000) Cytotoxicity and antioxidant activity of Curcumin I, II and III in Curcumin longa. Phytomedicine 7, 303-308
  40. Rebak J, Gryglewski RJ. (1988) Flavonoids and scavengers of superoxide anion. Biochem. Pharmacol. 37, 83-88 https://doi.org/10.1016/0006-2952(88)90169-4
  41. Refsness K, Olsnes S, Phil A. (1974) Toxic proteins Abrin (Abrus precatorius) and Ricin studies of their binding to and entry into Ehrlich ascites cells. J. BioI. Chem. 249, 3557-3562
  42. Robak J, Gryglewski RJ. (1996) Bioactivity of flavonoids. Pol. J. Pharmacol. 48, 555-564
  43. Rosangkima G, Prasad SB. (2004) Antitumour activity of some plants from Meghalaya and Mizoram against murine ascites Dalton's lymphoma. Indian J. Exp. BioI. 2, 981-988
  44. Sakagami H, Ikeda M, Unten S, Takeda K, Murayama Jl, Hamada A, Kimura K, Komatsu N, Konno K. (1987) Antitumour activity of polysaccharide fractions from pinecone extract of Pinus pavifloa Sieb Et Zucc. Anticancer Res. 7, 1153-1159
  45. Sheeja KR., Kuttan G, Kuttan R. (1997) Cytotoxic and antitumour activity of Beriberin. Amala Res. Bull. 17, 73-76
  46. Siegas CP, Steffer B, Roble A, Pantz P. (1999) The effect of garlic preparation against tumor cell proliferation. Phytomedicine 6, 7-11
  47. Sivakumar T, R Sambath, Kumar R, Perumal P, Vamsi MLM, Sivakumar P, Kanagasabai R, Baskaran MV, Karki SS, Mazumder UK, Gupta M.(2005) Antitumor and antioxidant activities of Bryonia laciniosa against Ehrlich's Ascites Carcinoma bearing Swiss albino mice. Orient. Pharm. Exp. Med. 5, 322-330
  48. Sllchenmyer WJ, Von Hoff DD. (1991) Taxol: A new and effective anticancer drug (Review). Anticancer Druge 2, 519-530
  49. Stefani ED, Boffetta P, Deneo-Pellegrini H, Mendilaharsu M, Carzoglio JC, Ronco A, Olivera L. (1999) Dietary antioxidants and lung cancer risk: a case- control study in Uruguay. Nutr. Cancer 34, 100-110
  50. Sur P, Ganguly DK. (1994) Tea plant root extract an antineoplastic agent. Planta Med. 60, 106-109
  51. Syiem D, Syngai C, Kharbuli B, Kayang H, Khonguwir BS. (2003) Antitumour activity of crude root extract of Potentilla fulgens. Indian Drugs 40, 124-125
  52. Weber G, Shen F, Yeh YA. (1996) Increased signal transduction activity and down regulation in human cancer cells. Anticancer Res. 16, 3271-3273
  53. Williams LAD, Gossell-Williams M, Sajabi A, Barton EN, Fleischhacker R. (1997) Angiotensin converting enzyme inhibiting and anti-ipsogenic activities of Euphorbia hirta. Phytother. Res. 11, 401-402 https://doi.org/10.1002/(SICI)1099-1573(199708)11:5<401::AID-PTR119>3.0.CO;2-T
  54. Wintrobe MM, Lee GR, Boggs DR, Bithel TC, Athens JW, Foerester J. (1961) In: Clinical Hematology, 5th edition, Lea and Febiger., Philadelphia, pp. 326
  55. Workman P, Koy SB. (2001) Translating basic cancer research into new cancer therapeutics. A Trend Guide to Cancer Therapeutic 8, S1-S6 https://doi.org/10.1016/S1471-4914(02)02319-5
  56. Yoshikawa T, Kokura S, Tainika K, Naito Y, Kondo M. (1995) A novel cancer therapy based on oxygen radicals. Cancer Res. 55, 1617-1620