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Identification of Tumor Antigens in Lung Cancer Patient by SEREX

폐암 환자에서 면역항원유전자의 혈청학적 동정

  • Min, Young-Ki (Department of Biochemistry, School of Medicine, Pusan National University) ;
  • Ha, Jin-Mok (Department of Biochemistry, School of Medicine, Pusan National University) ;
  • Son, Young-Ok (Department of Biochemistry, School of Medicine, Pusan National University) ;
  • Park, Hae-Rim (Department of Biochemistry, School of Medicine, Pusan National University) ;
  • Lee, Min-Ki (Department of Internal Medicine, School of Medicine, Pusan National University) ;
  • Park, Yeoung-Min (Department of Microbiology and Immunology, School of Medicine, Pusan National University) ;
  • Kim, Cheol-Min (Department of Biochemistry, School of Medicine, Pusan National University) ;
  • Lee, Sang-Yull (Department of Biochemistry, School of Medicine, Pusan National University)
  • 민영기 (부산대학교 의과대학 생화학 교실) ;
  • 하진목 (부산대학교 의과대학 생화학 교실) ;
  • 손영옥 (부산대학교 의과대학 생화학 교실) ;
  • 박해림 (부산대학교 의과대학 생화학 교실) ;
  • 이민기 (부산대학교 의과대학 호흡기내과) ;
  • 박영민 (부산대학교 의과대학 미생물 및 면역학교실) ;
  • 김철민 (부산대학교 의과대학 생화학 교실) ;
  • 이상률 (부산대학교 의과대학 생화학 교실)
  • Published : 2007.08.30

Abstract

Serological anlysis of recombinant cDNA expression libraries (SEREX) has led to identification of several categories of new antigens recognized by the immune system of cancer patients, which are referred to as the cancer immunome. We analyzed normal testis cDNA expression libraries with serumobtained from non-small lung cancer patient and isolated 40 distinct antigen designated KP-LuT-1 through KP-LuT-40. Among these antigens 20 antigens were previously identified by SEREX analysis of other tumor types, and 20 out of 40 antigens (50%) did not match entries in Cancer Immunome Database and were considered newly identified antigens. Sequencing analysis showed that the anti-gens comprised 26 functional known proteins and 14 noble/uncharacterized gene products. Of these, the hypothetical protein KP-LuT-6 was shown tissue-restricted. RT-PCR showed it to be expressed strongly only in normal testis. In addition to normal tissues-restricted expression, KP-LuT-6 mRNA was detected in lung tumor samples(3/l0), stomach tumor samples(3/l0), and breast tumor samples(l/5), whereas not detected in colon tumor samples(O/I2). These data suggest that KP-LuT-6 is a cancer/testis (CT)-like antigen as a potential target for cancer immunotherapies.

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