Cytoprotective effect of polysaccharide isolated from different mushrooms against 7-ketocholesterol induced damage in mouse liver cell line (BNL CL. 2)

  • Kim, Joo-Shin ;
  • Chung, Hau Yin ;
  • Na, Keun
  • Published : 2007.09.20

Abstract

Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At $80\;{\mu}g/mL$ of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of $200\;{\mu}g/mL$ of each polysaccharide isolate to the cell line containing $80\;{\mu}g/mL$ of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.

Keywords

7-ketocholesterol;cytotoxicity;mushroom;polysaccharide

References

  1. Choi MY, Jung TY & Hahm KJ (1995). Cytotoxic effect of hot water soluble poly-saccharides from mushroom: Lentinus edodes and vitamin A & E supplementation against P388 cells. Korean Journal of Nutrition 28:1091-1099
  2. Ghelli A, Porcelli AM, Zanna C & Rugolo M (2002). 7-ketocholesterl and staurosporine induce opposite changes in intracellular pH, associated with distinct type of cell death in ECV304 cells. Arch Biochem Biophys 402:208-217 https://doi.org/10.1016/S0003-9861(02)00085-1
  3. Kewon MH, Jang H, Lim WJ, Chang H I, Kim CW, Yang HC, Hwang HJ & Sung HC (1999). Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom, Pleurotus Ostreatus. J Microbiol Biotechnol 9:450-456
  4. Smith LL (1981). Distribution of autoxidaiton properties. In: Cholesterol Autoxidation, p.49-119. Plenum Press, New York and London. USA
  5. Carmichael, J, DeGraff, WG, Gazdar AF, Minna JD & Mitchell JB (1987). Evaluation of a tetrazolium-based semiautomated colorimetric Assay: Assessment of chemosensitivity testing. Cancer Res 47:936-942
  6. Liu F, Ooi VEC & Chang ST (1997). Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60:763-771 https://doi.org/10.1016/S0024-3205(97)00004-0
  7. Peng SK, Tham P, Taylor CB & Mikkelson B (1997). Cytotoxicity of oxidation derivatives of cholesterol on culturedarotic smooth muscle cells and their effect on cholesterol biosynthesis. Am J Clin Nutr 32:1033-1039
  8. Sporri S, Chopra V, Egger N, Hawkins HK, Motamedi M, Dreher E & Schneider H (2001). Effect of 5-aminolaevulnic acid on human ovarian cancer cells and human vascular endothelial cells in vitro. Photochem Photobiol 64:8-20 https://doi.org/10.1016/S1011-1344(01)00179-8
  9. Lyons MA & Brown AJ (1999). Molecules in focus: 7-Ketocholesterol. Int J Biochem Cell Biol 31:369-375 https://doi.org/10.1016/S1357-2725(98)00123-X
  10. Woo MS (1983). Studied on antitumor components of Flammulina velutipes of Korea (II). Korean Journal of Mycology 11:147-150
  11. Kim J-S, Godber JS, King JM & Prinywiwatkul W (2001). Inhibition of cholesterol autoxidation by the nonsaponifiable fraction in rice bran in aqueous model system. J Am Oil Chem Soc 78:685-689 https://doi.org/10.1007/s11746-001-0326-8