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Production of Keratinolytic Protease by Bacillus pumilus RS7 and Feather Hydrolysate As a Source of Amino Acids

Bacillus pumilus RS7에 의한 난분해성 케라틴 분해효소의 생산 및 아미노산 공급원으로서 우모 분해산물

  • Woo, Eun-Ok (Department of Microbiology, Pusan National University) ;
  • Kim, Min-Ju (Department of Microbiology, Pusan National University) ;
  • Son, Hyeng-Sik (Department of Microbiology, Pusan National University) ;
  • Ryu, Eun-Youn (Department of Microbiology, Pusan National University) ;
  • Jeong, Seong-Yun (Korea bio-IT Foundry Center, Pusan National University) ;
  • Son, Hong-Joo (School of Applied Life Science, Pusan National University) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University) ;
  • Park, Geun-Tae (Research & University-Industry Cooperation. Pusan National University)
  • 우은옥 (부산대학교 미생물학과) ;
  • 김민주 (부산대학교 미생물학과) ;
  • 손형식 (부산대학교 미생물학과) ;
  • 유은연 (부산대학교 미생물학과) ;
  • 정성윤 (부산대학교 한국 Bio-IT 파운드리 센타) ;
  • 손홍주 (부산대학교 생명응용과학부) ;
  • 이상준 (부산대학교 미생물학과) ;
  • 박근태 (부산대학교 산학협력단)
  • Published : 2007.10.31

Abstract

Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates, The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2 6H2O 0.01%, respectively. The optimal temperature and initial pH was $30^{\circ}C$ and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $113.8\;{\mu}g/ml$ and $504.9\;{\mu}g/ml$, respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $47.2\;{\mu}g/ml$ and $334.0\;{\mu}g/ml$, respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.

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