Ultra-Rapid Real-Time PCR for the Detection of Human Immunodeficiency Virus (HIV)

Ultra Rapid Real-Time PCR에 의한 Human Immunodeficiency Virus (HIV)의 신속진단법

  • 이동우 (경기대학교 자연과학대학 생명과학과) ;
  • 김을환 (경기대학교 자연과학대학 생명과학과) ;
  • 유미선 (경기대학교 자연과학대학 생명과학과) ;
  • 한상훈 (경기대학교 자연과학대학 생명과학과) ;
  • 윤병수 (경기대학교 자연과학대학 생명과학과)
  • Published : 2007.06.30

Abstract

For the detection of Human Immunodeficiency Virus (HIV), multiple and ultra-rapid real-time PCR methods were developed. The target DNA sequences were deduced from HIV-1 specific 495bp partial env gene (gi_1184090) and from HIV-2 specific 294 bp partial env gene (gi_1332355), and were synthesized by using PCR-based gene synthesis on the reason of safety. Ultra-rapid real-time PCR was performed by $Genspector^{TM}$ using microchip-based, $1\;{\mu}l$ of reaction volume with extremely short time in each 3 step in PCR. The detection including DNA-amplification and melting temperature analysis was completed inner 15 minutes. The HIV-1 specific 117 bp-long and HIV-2 specific 119 bp-long PCR products were successfully amplified from minimum of template,2.3 molecules of each env gene. This kind of real-time PCR was designated as ultra-rapid real-time PCR in this study and it could be applied not only an alternative detection method against HIV, but also other pathogens using PCR-based detection.

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