The Effect of Bamboo (Phyllostachys nigra var. henenis Strapf) Leaf Extract on Ultraviolet B-induced Skin Damages in Mouse

자외선 B 조사 마우스에서 피부손상에 대한 분죽 (Phyllostachys nigra var. henenis Strapf)잎 추출물의 효과

  • Chae, Se-Lim (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-June (College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Jang, Jong-Sik (Department of Animal Science, Sngju National University) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute, KAERI) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • 채세림 (전남대학교 수의과대학) ;
  • 이해준 (전남대학교 수의과대학) ;
  • 문창종 (전남대학교 수의과대학) ;
  • 김종춘 (전남대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학) ;
  • 강성수 (전남대학교 수의과대학) ;
  • 장종식 (상주대학교 축산학과) ;
  • 조성기 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김성호 (전남대학교 수의과대학)
  • Published : 2007.06.30

Abstract

The effects of bamboo (Phyllostachys nigra var. henenis Strapf) leaf extract (BLE) on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1-hr or ICR mouse were investigated. The mice were treated with UVB ($200mJ/cm^2$) and were sacrificed 24 hours later. BLE (50 mg/kg of body weight) or vehicle (saline) was given i.p. at 36 and 12 hours before irradiation, and 30 minutes after irradiation. BLE cream (0.2%) or cream base (vehicle) was also topically treated at 24 hours and 15 minutes before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/cm length of epidermis, and 24 hours after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was significantly reduced by intraperitoneal injection (59.0%) and topical application (31.8%) of BLE extract. The numbers of DC in normal ICR mouse were $628.00{\pm}51.56\;or\;663.20{\pm}62.58\;per\;mm^2$ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive $cells/mm^2$ were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. The frequency of UVB ($200mJ/cm^2$)-induced DC decrease was reduced by treatment of BLE as 25.7% in i.p. group and 3.2% in topical application group compared with the irradiation control group. The results presented herein that BLE administration could reduce the extent of skin damages produced by UVB.

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