Utilization of Hypothalamic Hormones for Maturational Induction in Seawater Chum Salmon, Oncorhynchus keta

해면연어의 성숙을 유도하기 위한 시상하부호르몬의 이용

  • Park, Woo-Dong (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Lee, Cheul-Ho (Yeongdong Inland Fisheries Research institute, National Fisheries Research and Development Institute) ;
  • Shon, Young-Chang (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 박우동 (강릉대학교 해양생명공학부) ;
  • 이철호 (국립수산과학원 영동내수면연구소) ;
  • 손영창 (강릉대학교 해양생명공학부)
  • Published : 2007.05.31

Abstract

Freshwater-adapted maturing chum salmon (Oncorhynchus keta) involuntarily captured by stationary nets in Yang-yang seashore areas were transferred to freshwater in an outdoor raceway tank at Yeongdong Inland Fisheries Research Institute, NFRDI, Yang-yang, Gangwon, Korea and kept over 1 day until the start of the experiments. The freshwater-adapted females were single-injected intraperitoneally with gonadotropin-releasing hormone analogue, (GnRH-a: $70\;{\mu}g/kg$ body weight, BW) alone or combined with a dopamine receptor antagonist, pimozide($700\;{\mu}g/kg$ BW). Although gonadosomatic indices [GSI, (gonad weight/BW)${\times}100$] did not show significant changes in both 2004 and 2005, GSI of GnRH-a-injected fish during the 2005 trial slightly increased on the 5th and 7th days post-injection compared to those of vehicle treated fish. Hepatosomatic indices [HSI, (liver weight/BW)${\times}100$] of fish injected with GnRH-a alone and combined with pimozide decreased significantly on the 7th day post-injection in 2004(P<0.05). In 2005 trials, HSI was significantly reduced in GnRH-a treated fish on the 7th day post-injection (P<0.05). Pimozide-injected fish showed a pattern with increase of GSI and decrease of HSI, without significant differences. Taken together, these results suggest that at least in part hypothalamic hormones and dopamine receptor antagonist may induce sexual maturation in freshwater-adapted maturing chum salmon. It remains to evaluate these preliminary results by further researches.

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