DOI QR코드

DOI QR Code

Detection of Oyster-Associated Norovirus by Microchip Electrophoresis of an Amplified cDNA - Research Note -

  • Oh, Ho-Kyung ;
  • Sin, Yeong-Min ;
  • Kim, Ki-Hyun ;
  • Park, Kun-Sang ;
  • Kim, Dae-Byung ;
  • Ahn, Byung-Yoon ;
  • Kim, Ok-Hee
  • Published : 2007.06.30

Abstract

Noroviruses, members of the family Caliciviridae, are often found in shellfish grown in polluted water and are emerging as a leading cause of foodborne disease worldwide. As the presence of norovirus in food commodities becomes an important medical and social issue, there are increasing needs for designing improved detection methods for the virus. In this study, we tested the Agilent 2100 Bioanalyzer for the analysis of norovirus DNA amplified from oyster samples. Microchip electrophoresis provided us with more accurate information, compared to conventional agarose gel electrophoresis, in the resolution and quantification of amplified products. The development of an improved method for food-associated noroviruses would contribute to a rapid identification of contaminated food and improve our understanding of the modes of food contamination and norovirus transmission.

Keywords

norovirus;viral gastroenteritis;RT-PCR;bioanalyzer;microchip electrophoresis;oysters

References

  1. Koopmans M, Duizer E. 2004. Foodborne viruses: an emerging problem. Int J Food Microbiol 90: 23-41 https://doi.org/10.1016/S0168-1605(03)00169-7
  2. Koopmans M, von Bonsdorff CH, Vinje J, de Medici D, Monroe S. 2002. Foodborne viruses. FEMS Microbiol Rev 26: 187-205 https://doi.org/10.1111/j.1574-6976.2002.tb00610.x
  3. Kageyama T, Shinohara M, Uchida K, Fukushi S, Hoshino FB, Kojima S, Takai R, Oka T, Takeda N, Katayama K. 2004. Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to norovirus in Japan. J Clin Microbiol 42: 2988-2995 https://doi.org/10.1128/JCM.42.7.2988-2995.2004
  4. Vinje J, Vennema H, Maunula L, von Bonsdorff CH, Hoehne M, Schreier E, Richards A, Green J, Brown D, Beard SS, Monroe SS, de Bruin E, Svensson L, Koopmans MP. 2003. International collaborative study to compare reverse transcriptase PCR assays for detection and genotyping of noroviruses. J Clin Microbiol 41: 1423-1433 https://doi.org/10.1128/JCM.41.4.1423-1433.2003
  5. Jothikumar N, Lowther JA, Henshilwood K, Lees DN, Hill VR, Vinje J. 2005. Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples. Appl Environ Microbiol 71: 1870-1875 https://doi.org/10.1128/AEM.71.4.1870-1875.2005
  6. Kageyama T, Kojima S, Shinohara M, Uchida K, Fukushi S, Hoshino FB, Takeda N, Katayama K. 2003. Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription- PCR. J Clin Microbiol 41: 1548-1557 https://doi.org/10.1128/JCM.41.4.1548-1557.2003
  7. Traore O, Arnal C, Mignotte B, Maul A, Laveran H, Billaudel S, Schwartzbrod L. 1998. Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and polio-virus in experimentally contaminated mussels: comparison of several extraction and concentration methods. Appl Environ Microbiol 64: 3118-3122
  8. Kingsley DH, Meade GK, Richards GP. 2002. Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness. Appl Environ Microbiol 68: 3914-3918 https://doi.org/10.1128/AEM.68.8.3914-3918.2002
  9. Lees DN, Henshilwood K, Green J, Gallimore CI, Brown DW. 1995. Detection of small round structured viruses in shellfish by reverse transcription-PCR. Appl Environ Microbiol 61: 4418-4424
  10. Nishida T, Kimura H, Saitoh M, Shinohara M, Kato M, Fukuda S, Munemura T, Mikami T, Kawamoto A, Akiyama M, Kato Y, Nishi K, Kozawa K, Nishio O. 2003. Detection, quantitation, and phylogenetic analysis of noroviruses in Japanese oysters. Appl Environ Microbiol 69: 5782-5786 https://doi.org/10.1128/AEM.69.10.5782-5786.2003
  11. Lees D. 2000. Viruses and bivalve shellfish. Int J Food Microbiol 59: 81-116 https://doi.org/10.1016/S0168-1605(00)00248-8
  12. Ando T, Noel JS, Fankhauser RL. 2000. Genetic classification of Norwalk-like viruses. J Infect Dis 181 (Suppl 2): S336-348 https://doi.org/10.1086/315589
  13. Atmar RL, Estes MK. 2001. Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses. Clin Microbiol Rev 14: 15-37 https://doi.org/10.1128/CMR.14.1.15-37.2001
  14. Lu CY, Tso DJ, Yang T, Jong YJ, Wei YH. 2002. Detection of DNA mutations associated with mitochondrial diseases by Agilent 2100 bioanalyzer. Clin Chim Acta 318: 97-105 https://doi.org/10.1016/S0009-8981(01)00809-9
  15. Kojima S, Kageyama T, Fukushi S, Hoshino FB, Shinohara M, Uchida K, Natori K, Takeda N, Katayama K. 2002. Genogroup-specific PCR primers for detection of Norwalk-like viruses. J Virol Methods 100: 107-114 https://doi.org/10.1016/S0166-0934(01)00404-9
  16. Le Guyader F, Haugarreau L, Miossec L, Dubois E, Pommepuy M. 2000. Three-year study to assess human enteric viruses in shellfish. Appl Environ Microbiol 66: 3241-3248 https://doi.org/10.1128/AEM.66.8.3241-3248.2000
  17. Jean J, D'Souza DH, Jaykus LA. 2004. Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods. Appl Environ Microbiol 70: 6603-6610 https://doi.org/10.1128/AEM.70.11.6603-6610.2004