강낭콩 유식물로부터 분리한 Lectin의 생화학적 특성

Biochemical Characterization of Lectin Purified from Kidney Bean Seedling

  • 발행 : 2007.02.28

초록

강낭콩 유식물로부터 PBS에 의한 추출, $(NH_4)_2SO_4$ 침전, Sepadex G-100 column chromatography에 의해 lectin을 분리한 다음, 이들의 생화학적 특성으로서 분자량, 적혈구 응집반응, 열 안정성, 최적 온도 및 최적 pH를 연구하였다. 이 과정에 토끼 혈액의 적혈구를 이용하여 활성을 측정하였다. 이 lectin의 분자량은 46 kDa와 44 kDa로서, 각각 2개의 subunit를 갖는 tetramer이다. 정제된 이 lectin의 최적 반응 온도는 30$^{\circ}C$이며, $40\sim80^{\circ}C$에서 열 안정성을 보였다. 또한 이 lectin의 최적 pH는 pH 8.2이다.

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