Effect of M11C (Non-lectin Components) Obtained from Korean Mistletoe on the $IL-1\beta$ Secretion from Mouse Splenocytes

쥐의 비장세포로부터 $IL-1\beta$ 분비에 있어서 한국산 겨우살이 추출물 M11C (비렉틴 구성물질)의 효과

  • Jun, Myung-Ha (Department of Immunology, College of Medicine, Konkuk University) ;
  • Kang, Tae-Bong (The Institute for Biomedical Research, Handong University) ;
  • Chang, Sung-Ho (Department of Immunology, College of Medicine, Konkuk University) ;
  • Choi, Wahn-Soo (Department of Immunology, College of Medicine, Konkuk University) ;
  • Seong, Nak-Sul (National Crop Experiment Station, RDA) ;
  • Her, Erk (Department of Immunology, College of Medicine, Konkuk University)
  • 전명하 (건국대학교 의과대학 면역학교실) ;
  • 강태봉 (한동대학교 생의학 연구소) ;
  • 장성호 (건국대학교 의과대학 면역학교실) ;
  • 최완수 (건국대학교 의과대학 면역학교실) ;
  • 성낙술 (농촌진흥청 작물시험장 특용작물과) ;
  • 허억 (건국대학교 의과대학 면역학교실)
  • Published : 2007.02.28

Abstract

Korean mistletoe (Viscum album L) extract has been found to posses immunoregulating activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract activates splenocytes to secret interleukin $1\beta(IL-1\beta)$. The splenocytes were treated with M11C, and then collected the supernatant and cell lysate that were prepared to analyze the level of $IL-1\beta$, using ELISA, immunoblotting, and RT-PCR. Maximum effective dose and time of M11C on $IL-1\beta$ secretion from splenocytes were $200{\mu}g/m\ell$ and 8 hours, respectively. Treatment dose and time for the maximum expression of $IL-1\beta$ mRNA were $200{\mu}g/m\ell$ and 4 hours, respectively. Saccharide degradation enzyme Viscozyme L completely blocked the effect of M11C on $IL-1\beta$ secretion from splenocytes. As the result, among non-lectin components saccharide could be regarded as a main component for $IL-1\beta$ expression from splenocytes.

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