D-amino Acid Oxidase (DAO) Gene as a Novel Selection Marker for Plant Transformation

새로운 선발 마커 D-아미노산 산화효소 유전자를 이용한 식물 형질전환

  • Lim, Sun-Hyung (National Institute of Agricultural Biotechnology, RDA) ;
  • Woo, Hee-Jong (National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Si-Myung (National Institute of Agricultural Biotechnology, RDA) ;
  • Jin, Yong-Moon (National Institute of Agricultural Biotechnology, RDA) ;
  • Cho, Hyun-Suk (National Institute of Agricultural Biotechnology, RDA)
  • 임선형 (농촌진흥청 농업생명공학연구원) ;
  • 우희종 (농촌진흥청 농업생명공학연구원) ;
  • 이시명 (농촌진흥청 농업생명공학연구원) ;
  • 진용문 (농촌진흥청 농업생명공학연구원) ;
  • 조현석 (농촌진흥청 농업생명공학연구원)
  • Published : 2007.03.31


Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene. $T_{1}$ tobacco seeds derived $T_{0}$ tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.



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