DOI QR코드

DOI QR Code

Effects of Caprylic Acid and Cyclodextrin Complex on In vitro Fermentation Characteristics and Methane Production

Caprylic Acid와 Cyclodextrin 복합물이 In vitro 반추위 발효성상 및 메탄 생성에 미치는 영향

  • Kim, K.H. (National Institute of Animal Science, RDA) ;
  • Seol, Y.J. (National Institute of Animal Science, RDA) ;
  • Lee, S.S. (Department of Dairy science, College of Agriculture and Science, Gyeong Sang National University) ;
  • Oh, Y.G. (National Institute of Animal Science, RDA) ;
  • Nam, I.S. (National Institute of Animal Science, RDA) ;
  • Kim, D.H. (National Institute of Animal Science, RDA) ;
  • Choi, C.W. (National Institute of Animal Science, RDA)
  • 김경훈 (농촌진흥청 축산과학원) ;
  • 설용주 (농촌진흥청 축산과학원) ;
  • 이성실 (경상대학교 농생명학부 낙농학전공) ;
  • 오영균 (농촌진흥청 축산과학원) ;
  • 남인식 (농촌진흥청 축산과학원) ;
  • 김도형 (농촌진흥청 축산과학원) ;
  • 최창원 (농촌진흥청 축산과학원)
  • Published : 2007.10.31

Abstract

This study was conducted to evaluate the effects of dietary addition of caprylic acid(CA)-cyclodextrin (CD) complex on in vitro fermentation characteristics, total gas and methane production. Experiment was done with six treatment groups; 1) no CA-CD complex(control), 2) CA 20 mg(T1), 3) CD 830 mg(T2), 4) CA-CD complex 425 mg(T3), CA-CD complex 850mg(T4), CA-CD complex 1,700 mg(T5). Ruminal pH, ammonia and total VFA concentrations of T2, T3, T4 and T5 were lower(P<0.05) than those of control and T1 for the 12h incubation. The increase in molar percentage of propionate was observed in T4 and T5 compared with control and T2 for the 8h incubation(P<0.05), however, the ratio of acetate to propionate was unchanged in all treatments. Total gas of T1 was lower than that of control, but T2, T3, T4 and T5 were higher compared with control for 12h incubation(P<0.05). If the methane ratio (as %) to total gas for all treatments was compared, T3, T4 and T5(CA-CD supplemented groups) averaged 2.7% whereas control, T1 and T2 showed 3.4, 2.8 and 5.1%, respectively. Therefore, according to these results, it might be concluded that supplementation of CA-CD complex could reduce methane production without disrupting ruminal fermentation.

Keywords

Caprylic acid;Cyclodextrin;Methane;Rumen

References

  1. Ajisaka, N., Mohammed, N., Hara, K., Mikumi, K., Hara, K., Hashimoto, Kumata, T., Kanda, S. and Itabashi, H. 2002. Effects of medium-chain fatty acid-cyclodextrin complexes on ruminal methane production in vitro. Anim. Sci. J. 73:479-484 https://doi.org/10.1046/j.1344-3941.2002.00066.x
  2. Beauchemin, K. A. and MeGinn, S. M. 2006. Methane emission from beef cattle: Effects of fumaric acid, essential oil, and canola oil. J. Anim. Sci. 84:1489-1496
  3. Chaney, A. L. and Marbach, E. P. 1962. Modification reagents for determination of urea and ammonia. Clin. Chem. 8:130-132
  4. Dochene, D., Bochot, A., Yu, S. C., Pepin, C. and Seiller, M. 2003. Cyclodextrin and emulsion. Int. J. Pharm. 266:85-90 https://doi.org/10.1016/S0378-5173(03)00384-3
  5. Erwin, E. S., Marco D. J. and Emery, E. M. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1770 https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  6. Frumholtz, P. P., Newbold, C. J. and Wallace, R. J. 1989. Influence of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec). J. Agric. Sci. 113:169-172 https://doi.org/10.1017/S002185960008672X
  7. IPCC. 1996. Greenhouse gas inventory revised methodology. guidelines for national greenhouse gas inventories. Vol. 3. Bracknell. UK
  8. Johnson, K. A. and Johnson, D. E. 1995. Methane emissions from cattle. J. Anim. Sci. 73:2483-2492
  9. Lila Z. A., Mohammed, N., Kanda, S., Kamada, T. and Itabashi, H. 2003. Effect of ${\alpha}$-cyclodextrin allyl isothiocyanate on ruminal microbial methane production in vitro. Anim. Sci. J. 74:321-326 https://doi.org/10.1046/j.1344-3941.2003.00123.x
  10. Lila, Z. A., Mohammed, N., Tatsuoka (Ajisaka), N., Kanda, S., Kamada, T., Kurokawa, Y. and Itabashi, H. 2004. Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo. Anim. Sci. J. 75:15-22 https://doi.org/10.1111/j.1740-0929.2004.00149.x
  11. Machmuller, A. 2006. Medium-chain fatty acids and their potential to reduce methanogenesis in domestic ruminants. Agric. Ecosy. Environ. 112:107-114 https://doi.org/10.1016/j.agee.2005.08.010
  12. Martin Del Valle, E. M. 2004. Cyclodextrin and their uses: a review. Proc. Biochem. 39:1033-1046
  13. Miyazaki, K., Hino, T. and Itabashi, H. 1989. Changes caused by ethanol in fermentation pattern and membrane fatty acid composition of rumen microorganisms. Jpn. J. Zootech Sci. 60:776-782
  14. Newbold, C. J., Lassalas, B. and Jouany, J. P. 1995. The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Lett. Appl. Microbiol. 21:230-234 https://doi.org/10.1111/j.1472-765X.1995.tb01048.x
  15. SAS User's Guide: Statistics, Version 9.1 Edition. 2002. SAS Inst., Inc., Cary, NC
  16. Soliva, C. R., Meile, L., Hindrichsen, I. K., Kreuzer, M. and Machmuller, A. 2004. Myristic acid supports the immediate inhibitory effect of lauric acid on ruminal methanogens and methane release. Anaerbe. 10:260-276
  17. The Govemment of the Republic of Korea. 2003. Second National Communication of the Republic of Korea Under the United Nations Framework Convention on Climate Change. http://www.keei.re.kr
  18. Van Nevel, C. J. and Demeyer, D. I. 1992. Influence of antibiotics and a deaminase inhibitor on volatile fatty acids and methane production from detergent washed hay and soluble starch by rumen microbes in vitro. Anim. Feed Sci. Technol. 37:21-31 https://doi.org/10.1016/0377-8401(92)90117-O
  19. Mohammed, N. and Lila, Z. A. 2004. Effects of cyclodextrin-iodopropane complex on methane production, ruminal fermentation and microbes, digestibility and blood metabolites in steers. Anim. Sci. J. 75:131-137 https://doi.org/10.1111/j.1740-0929.2004.00167.x
  20. Czerkawski, J. W., Blaxter, K. L. and Wainman, F. W. 1966. The metabolism of oleic, linoleic and linolenic acids by sheep with reference to their effects on methane production. Br. J. Nutr. 20:349-362 https://doi.org/10.1079/BJN19660035