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Analysis of Single Nucleotide Polymorphisms of Leptin Gene in Hanwoo(Korean Cattle)

한우 Leptin 유전자의 단일 염기 다형성 분석

  • Lee, J.-Min (Department of Animal Biotechnology, Konkuk University) ;
  • Song, G.C. (Department of Animal Biotechnology, Konkuk University) ;
  • Lee, J.Y. (National Genome Research Institute, Korea National Institute of Health) ;
  • Kim, Young-Bong (Department of Animal Biotechnology, Konkuk University)
  • 이정민 (건국대학교 동물생명공학과) ;
  • 송기철 (건국대학교 동물생명공학과) ;
  • 이종영 (국립보건연구원 유전체센터) ;
  • 김영봉 (건국대학교 동물생명공학과)
  • Published : 2007.06.30

Abstract

Leptin, the product of the obese(ob) gene, is an adipocyte-derived hormone for the regulation of whole- body energy storage and energy usage. It has been reported that the homozygous mutations in the gene for leptin(LEP) induce obesity and reduce energy expenditure. In cattle, LEP has significant roles directly or indirectly related with phenotypes such as body weight and fat deposits, therefore SNPs of LEP have been considered important genetic marker to estimate carcass fat content in cattle. In this study, SNPs were screened in LEP(2,222 bp) between intron 1 to 3'-UTR from 24 independent Hanwoo(Korean cattle) by PCR and DNA sequencing. Total 25 SNPs were found and two nonsynonymous SNPs including T1163A(V19E) and G3256A(G132D) were newly detected only from Hanwoo. Among 20 SNPs previously reported in cattle, 16 SNPs were found in Hanwoo; however, the frequencies of some SNPs were significantly different between Hanwoo and western cattle breeds. The other 4 SNPs were not detected from Hanwoo. These Hanwoo specific SNP patterns in LEP will be used in development of molecular marker and application to genetic improvement of Hanwoo.

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