Detection of Copy Number Variation of the KIT Gene in the Landrace Breed using an Quantitative Oligonucleotide Ligation Assay(qOLA)

Quantitative Oligonucleotide Ligation Assay(qOLA)를 이용한 Landrace 품종의 KIT 유전자 반복수 변이 탐지

  • Seo, B.Y. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University) ;
  • Kim, J.H. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University) ;
  • Nahm, D.W. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University) ;
  • Yoo, C.K. (Division of Agriculture and Life, Gyeongsang National University) ;
  • Lee, S.H. (Gyeongnam Animal Science Technology) ;
  • Lee, J.B. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University) ;
  • Lim, H.T. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University) ;
  • Jung, E.J. (Division of Agriculture and Life, Gyeongsang National University) ;
  • Cho, I.C. (National Institute of Subtropical Agriculture, R. D. A.) ;
  • Heo, K.N. (National Institute of Animal Science, R. D. A.) ;
  • Jeon, J.T. (Division of Applied Life Science(BK21), Graduate School of Gyeongsang National University)
  • 서보영 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 김재환 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 남덕우 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 유채경 (경상대학교 농생명학부) ;
  • 이상호 (경남동물과학기술) ;
  • 이재봉 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 임현태 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 정은지 (경상대학교 농생명학부) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 허강녕 (축산과학원 동물유전체과) ;
  • 전진태 (경상대학교 대학원 응용생명과학부(BK21))
  • Published : 2007.10.31


Recently, copy number variations (CNV) of genes or genomic segments have been intensively studied and various analysis methods have been developed. In this study, quantitative oligonucleotide ligation assay (qOLA) was applied to investigate CNV of KIT gene in the Landrace breed. A combined assay using qOLA and pyrosequencing, 6 genotype classes, I1/I1 or I3/i (IBe), I1/I2 or I3/IP, I1/I3, I1/IP or I2/i (IBe), I2/I2and I2/IP, were identified from 44 Landrace pigs. Genotype assignment using grouping features of measurements on a scatter plot showed 100% agreement with those using a statistical assignment by PROC FASTCLUS procedure implemented in the SAS package. Two versions (3100 and 3130) of ABI sequencers gave the same genotyping results, indicating there was no influence on qOLA by different versions of instrument, however, the means of standard deviation and coefficient of variation from the qOLA on a ABI 3130 (2.33 and 4.10) was lower than those from the qOLA on a ABI 3100 (2.67 and 4.81). Effect of proteinase K treatment on the PCR product followed by qOLA was very clear because noise peaks were disappeared and the observed ration fit better to the reference ratio corresponding to each genotype.


Copy number variation;KIT;Landrace;OLA


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