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한우 ADSF/resistin 유전자의 단일 염기 다형과 육질관련형질 상관 분석

Analysis of the ADSF/resistin Gene Polymorphism Associated with Carcass Traits in Hanwoo

  • 박지애 (진주산업대학교 동물생명과학과) ;
  • 강혜경 (진주산업대학교 동물생명과학과) ;
  • 채은진 (진주산업대학교 동물생명과학과) ;
  • 서강석 (축산과학원) ;
  • 김상훈 (경희대학교 생물학과) ;
  • 윤철희 (서울대학교 식품동물생명공학부) ;
  • 문양수 (진주산업대학교 동물생명과학과)
  • Park, J.A. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kang, H.K. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Chae, E.J. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Seo, K.S. (National Institute of Animal Science, RDA) ;
  • Kim, S.H. (Department of Biology, Kyung Hee University) ;
  • Yun, C.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Moon, Y.S. (Department of Animal Science and Biotechnology, Jinju National University)
  • 발행 : 2007.10.31

초록

본 연구는 후대검정 한우 295두의 혈액으로부터 genomic DNA를 추출하여 PCR 방법에 의한 증폭과 염기서열 분석을 통하여 ADSF/resistin 유전자의 단일염기다형을 발굴하고 이들과 한우 육질관련형질과의 상관관계를 분석하기 위하여 실시하였다. 확보된 DNA로부터 염기서열을 결정한 결과 promoter와 4개의 exon영역에서는 SNP를 찾지 못하였으나 intron 영역에서 7개의 SNP를 발굴하였다. 발굴된 SNP의 출현 빈도는 0.027에서 0.16까지 그 차이가 많았다. 육질형질과의 상관분석에서 이들 SNP 중 intron 2에서 발굴된 764A ins 만이 근내지방도와 상관관계가 발견되었다(P<0.05). 근내지방도는 유전력이 매우 높기 때문에 이번에 발굴된 ADSF/resistin 유전자의 SNP 764A ins와 같이 유전표지인자를 이용하는 것이 근내 지방도의 개량을 위해 우수한 결과를 가지는 것으로 사료된다. 가축의 경제형질의 경우 다수의 유전자가 관여하기 때문에 한 개의 유전자를 이용한 가축의 선발 또는 개량에 이용한다는 것은 제한적일 수 있다. 따라서 다수의 관련 유전자를 이용한 다형현상과 경제형질과의 연관성 연구가 동반될 때 육질개선 및 가축개량에 실질적인 증대효과가 있을 것으로 사료된다.

키워드

ADSF/resistin;Hanwoo;Carcass trait;SNP

참고문헌

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