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Analysis of the ADSF/resistin Gene Polymorphism Associated with Carcass Traits in Hanwoo

한우 ADSF/resistin 유전자의 단일 염기 다형과 육질관련형질 상관 분석

  • Park, J.A. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kang, H.K. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Chae, E.J. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Seo, K.S. (National Institute of Animal Science, RDA) ;
  • Kim, S.H. (Department of Biology, Kyung Hee University) ;
  • Yun, C.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Moon, Y.S. (Department of Animal Science and Biotechnology, Jinju National University)
  • 박지애 (진주산업대학교 동물생명과학과) ;
  • 강혜경 (진주산업대학교 동물생명과학과) ;
  • 채은진 (진주산업대학교 동물생명과학과) ;
  • 서강석 (축산과학원) ;
  • 김상훈 (경희대학교 생물학과) ;
  • 윤철희 (서울대학교 식품동물생명공학부) ;
  • 문양수 (진주산업대학교 동물생명과학과)
  • Published : 2007.10.31

Abstract

Adipocyte-specific secretory factor(ADSF)/resistin, an hormone, is a small cysteine-rich protein secreted from adipose tissue and ADSF/resistin has been implicated in modulating adipogenesis in human and rodents. Although the exact role of ADSF/resistin in bovine has not been identified, it may have directly or indirectly involved in adipocyte differentiation. The objective of this study was to investigate its DNA polymorphism associated with carcass traits in Korean Native Cattle(Hanwoo). To investigate DNA polymorphism in Hanwoo ADSF/resistin gene, blood samples were taken from 295 Hanwoo steers belonging to progeny testing at Hanwoo Improvement Center in Korea. Seven single nucleotide polymorphisms(SNPs) were found in intron regions but not in any other regions including promoter (1.7kb) and 4 exons. The highest frequency among SNPs was C186A(0.16/0.84) following G964A (0.156/0.884). The significant correlation(P<0.05) between the SNPs and economic traits was found on 764Ains associated with marbling but not from any other SNPs determined.  A computer simulation was also conducted to assess the efficiency of marker assisted selection(MAS) versus the conventional breeding scheme.  Results revealed that MAS was more efficient as a breeding tool compared to the conventional. In conclusion, ADSF/Resistin gene is one of candidate genes to evaluate the quality, especially marbling score, in Hanwoo.

Keywords

ADSF/resistin;Hanwoo;Carcass trait;SNP

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