Effects of Cellulolytic Microbes Inoculation During Deep Stacking of Spent Mushroom Substrates on Cellulolytic Enzyme Activity and Nutrients Utilization by Sheep

버섯부산물 퇴적발효 시 섬유소 분해균 접종이 섬유소 분해성 효소 활력과 면양의 영양소 이용성에 미치는 영향

  • Kim, Y.I. (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Jun, S.H. (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Yang, S.Y. (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Huh, J.W (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Kwak, W.S. (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University)
  • 김영일 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 정세형 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 양시용 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 허정원 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 곽완섭 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공)
  • Published : 2007.10.31


This study was conducted to determine effects of cellulolytic microbes inoculation to sawdust-based spent mushroom substrate(SMS) during deepstacking on fermentation parameters, total microbial counts and cellulolytic enzyme activity and to on SMS nutrients utilization by sheep. For sheep metabolism trials, six sheep(ram, average 54.8kg) were fed a Control diet(70% concentrates, 15% rice straw and 15% SMS with no microbial treatment on a dry basis) and a Treatment diet(the same diet including SMS with a microbial treatment) for 2 trials. Spent mushroom substrates with or without a microbial(4 strains including 1 strain of Enterobacter ludwigii, 1 strain of Bacillus cereus and 2 strains of Bacillus subtillis) treatment (1% of SMS on wet basis) were deepstacked for 7 days. The internal temperatures in 1.2 M/T of SMS deepstacks reached to 50±5℃ within 7 days of storage. Total microbial counts remarkably decreased (P<0.05) with a deepstacking process and were not affected(P>0.05) by the microbial treatment. For fibrolytic enzyme activity, CMCase and xylanase activities were decreased(P<0.05) by a deepstacking process. After deepstacking, the microbial treatment showed about 2.5-times higher(P<0.05) for CMCase activity and about 4-times higher(P<0.05) for xylanase activity than those of the Control. Activities of ligninolytic enzymes such as laccase and MnP were not affected by the microbial treatment. The sheep fed the microbially treated SMS diet had a tendency of greater total tract digestibilities of ash(P=0.051), NFE (P=0.071), hemicellulose(P=0.087) and NDF(P=0.096) than those fed the untreated SMS diet. Nitrogen balance of sheep was not affected(P>0.05) by feeding of microbially treated SMS. Accordingly, these results indicate that cellulolytic microbes inoculation during deepstacking of SMS may improve the bio- utilization of SMS by sheep.


Spent mushroom substrates;Spent mushroom compost;Microbial treatment;Digestibility;Feed


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