DOI QR코드

DOI QR Code

The Effects of Negative- and Positive- Charged Surfactants on In vitro DM Digestibility and the Growth of Ruminal Mixed Microorganisms

양(+) 이온성 및 음(-) 이온성 계면활성제 첨가가 반추위 혼합 미생물에 의한 In vitro 건물소화율 및 미생물 성장에 미치는 영향

  • Lee, S.J. (Division of Applied Life Science, Gyeongsang National University) ;
  • Shin, N.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, W.Y. (Department of Animal Science, Korea National Agricultural College) ;
  • Moon, Y.H. (Department of Animal Sci. & Biotech., RAIC, Jinju National University) ;
  • Kim, H.S. (National Livestock Research Institute, R.D.A) ;
  • Ha, J.K. (School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, S.S. (Division of Applied Life Science, Gyeongsang National University)
  • 이신자 (경상대학교 응용생명과학부) ;
  • 신년학 (경상대학교 응용생명과학부) ;
  • 김완영 (한국농업대학 축산학과) ;
  • 문여황 (진주산업대학교 동물생명과학과) ;
  • 김현섭 (축산과학원 축산자원개발부) ;
  • 하종규 (서울대학교 농생명공학부) ;
  • 이성실 (경상대학교 응용생명과학부)
  • Published : 2007.10.31

Abstract

In order to investigate the effects of supplemental ionic surfactants in in vitro ruminal fermentation, N-Lauroylsarcosine sodium salt(N-LSS) and sodium dodecyl sulfate(SDS) for negative(-) ionic surfactant, and hexadecylpyridinium chloride monohydrate(HPCM) and hexadecyltrimethyl ammonium bromide(HTAB) for positive (+) ionic surfactant were supplemented by 0.05% and 0.1% into the Dehority’s artificial medium containing rice straw(1mm) as a substrate. In vitro DM digestibility, the growth of rumen mixed microbes, pH, cumulative gas production and SEM(Scanning Electron Microscopy) observation of microbial attachment on rice straw particle were investigated through the experiment composing 9 treatments (two supplemental levels of two positive ionic(+) surfactant, two supplemental levels of two negative(-) ionic surfactant) including the control. The sample collection was at 6, 12, 24, 48 and 72 h post fermentation with 3 replications per treatments. DM digestibility in treatments supplemented (+) or (-) surfactants almost stopped afterward 12 h fermentation, in vitro DM digestibility at 72 h post fermentation in the ionic surfactants was at half level of that of the control(P<0.05). Accumulative gas production in in vitro was less(P<0.05) with addition of ionic surfactants compared to the control. The amount of rumen mixed microbes recovered from in vitro incubation fluid pleateaued at 12 h post fermentation for the positive (+) ionic surfactants, but steadily increased as fermentation time elapsed for the control. Rumen microbial growth rate was significantly(P<0.05) low in the negative(-) ionic surfactant compared to the control. pH of the incubation fluid was ranged from 6.02 to 7.20, and was the highest in the negative(-) ionic surfactants, and was the lowest in the control(P<0.05). In SEM observation, rumen microbial population attached on rice straw particle was less with addition of ionic surfactants than the control. In conclusion we could not found any positive effects of negative- and positive- charged surfactants on rumunal fermentation characteristics and rumen microbial growth rates.

Keywords

Negative-ionic surfactant;Positive-ionic surfactant;Digestibility;SEM

References

  1. A.O.A.C. 1995. Official methods of analysis 16th edition. Association of official analytical chemists, Washington, D. C
  2. Busquet, M., Calsamiglia, A., Ferret and Kamel, C. 2006. Plant extracts affect in vitro rumen microbial fermentation. J. Dairy Sci. 89:761 https://doi.org/10.3168/jds.S0022-0302(06)72137-3
  3. De Oude, N. T. 1992. The handbook of environmental chemistry. Vol 3 part F: anthropogenic compounds. Springer Verlag, Heidelberg, FRG
  4. Dehority, B. A. and Scott, H. W. 1967. Extent of cellulose and hemicellulose digestion in various forage by pure cultures of rumen bacteria. J. Dairy Sci. 50:1136 https://doi.org/10.3168/jds.S0022-0302(67)87579-9
  5. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11:1 https://doi.org/10.2307/3001486
  6. Ferorak, P. M. and Hrwdey, S. E. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4:425 https://doi.org/10.1080/09593338309384228
  7. Helenius, A. and Simons, K. 1975. Solubilization of membranes by detergents. Biophys. Acta 415:29
  8. Ho, Y. W., Aldullah, N. and Jalaludin, S. 1988. Penetrating structures of anaerobic rumen fungi in cattle and swamp buffalo. J. Gen. Micobial. 134:177
  9. Hulme, M. A. and Stranks, D. W. 1970. Induction and the regulation of production of cellulase by fungi. Nature, London 226:469 https://doi.org/10.1038/226469a0
  10. Lang, S. and Wagner, F. 1993. Biological activities of biosurfactants. In: Kosaric Biosurfactants. Marcel Dekker, Inc. New York, USA
  11. Lee, S. S. and Ha, J. K. 2003. Influences of surfactants Tween 80 on the gas production, cellulose digestion activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16:1151 https://doi.org/10.5713/ajas.2003.1151
  12. Lee, S. S., Ahn, B. H., Kim, H. S., Kim, C. H., Cheng, K. J. and Ha, J. K. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16:104 https://doi.org/10.5713/ajas.2003.104
  13. Moore, J. E. 1970. Procedure for two-stage in vitro digestion of forage. In L. E. Harrison(ed.). Nutrition research technique for domestic and wild animals. J. Brit. Grassl. Sci. 18:119
  14. Munn, E. A., Hazlewood, G. P. and Graham, M. 1983. Uptake and incorporation of the products of proteolysis by the rumen bacterium Bacteroides ruminicola R8/4. Curr. Microbiol. 8:317 https://doi.org/10.1007/BF01573701
  15. National Research Council. 2001. Nutrient Requirements of Dairy Cattle. Natl. Acad. Press, Washington DC
  16. Reese, E. T. and Maguire, A. 1969. Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiol. 17:242
  17. SAS. 1999. SAS/STAT software for PC. Release 8.01. SAS institute Inc., Cary, N. C., U.S.A
  18. Yang, C. M. and Russell, J. B. 1993. The effect of monen supplementation on ruminal ammonia accumulation in vitro and the numbers of amino acid-fermenting bacteria. J. Anim. Sci. 71:3470
  19. Yazdi, M. T., Woodward, J. R. and Radford, A. 1990. The cellulase complex of Neurospora crassa: activity, stability and release. J. Gen. Microbiol. 136:1313 https://doi.org/10.1099/00221287-136-7-1313
  20. 이성실, 하종규, 강희신, Cheng, K. J. 1997. 반추 위 혐기성 곰팡이의 산업적 활용 방안에 관한 고찰 : 종설. 한국낙농학회지. 19:249